ABSTRACT The most widely used source of onion cytoplasmic male-sterility (CMS) is controlled by a single nuclear gene and a cytoplasmic factor, and the sterility trait can be recovered by the dominant nuclear gene (Ms). Marker-assisted selection (MAS) has a great potential in the selection of male sterility lines and maintainer lines. In this study, two markers at the Ms loci and one cytoplasm marker were used to a develop multiplexed polymerase chain reaction (PCR) system for MAS of fertility traits in onion. Seven pairs of PCR primers were designed on the basis of the flanking sequences (F1890 and S1887) of the male-fertility restoration (Ms) locus and a mitochondrial sequence (mt472) in onion. One set of compatible primers were selected to develop a multiplex-PCR molecular marker (AcCN), which was established in forty-eight lines. The fertility types of cytoplasm and nuclear were distinguished by one-time PCR detection. The accuracy of the AcCN marker was verified in practical backcross breeding population. The AcCN marker types perfectly matched with their genotypes and phenotypes. It can be concluded that this marker is of great practical significance for MAS in the selection of onion maintainer lines.

中文翻译：

---

基于多重 PCR 的洋葱 (Allium cepa L.) 雄性生育基因分型方法的开发

摘要 洋葱细胞质雄性不育（CMS）来源最广泛，由单一核基因和细胞质因子控制，不育性状可由显性核基因（Ms）恢复。标记辅助选择（MAS）在选择雄性不育系和保持系方面具有很大的潜力。在这项研究中，Ms 基因座的两个标记和一个细胞质标记被用于开发用于洋葱生育性状 MAS 的多重聚合酶链反应 (PCR) 系统。基于雄性生育恢复（Ms）基因座的侧翼序列（F1890和S1887）和洋葱中的线粒体序列（mt472）设计了七对PCR引物。选择了一组相容的引物来开发多重 PCR 分子标记 (AcCN)，该标记建立在 48 行中。通过一次性PCR检测区分细胞质和细胞核的生育类型。AcCN 标记的准确性在实际回交育种群体中得到验证。AcCN 标记类型与其基因型和表型完美匹配。可以得出结论，该标记对于MAS在洋葱保持系的选择中具有重要的现实意义。