ABSTRACT The YABBY gene family plays a vital role in the polarity development of lateral organs. Here, we isolated a BoYABBY1 (BoYAB1) gene from Bambusa oldhamii and encodes for 167aa protein. This gene contains a zinc-finger motif in its N terminus and a YABBY domain in its C terminus. Based on multiple sequence alignment, BoYAB1 was shown closely related to OsYAB1 from rice with a sequence identity of 88.76%. Expression analysis (RT-qPCR) and in-situ hybridisation results revealed that the preferential accumulation of BoYAB1 was in the stamen and carpel primordia of flower buds. Ectopic expression of BoYAB1 in Arabidopsis thaliana caused late flowering, narrow while curled leaves, and the curl was pointed towards the abaxial side. Further assays indicated that the observed phenotypic differences were associated with the expression level of exogenous BoYAB1 gene in transgenic plants. These results suggested that the BoYAB1 gene might be involved in leaf development and regulation of flowering time.

中文翻译：

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BoYAB1 是 Bambusa oldhamii YABBY 基因家族成员的异位表达，导致拟南芥卷叶和晚花

摘要 YABBY 基因家族在侧向器官的极性发育中起着至关重要的作用。在这里，我们从 Bambusa oldhamii 中分离出一个 BoYABBY1 (BoYAB1) 基因并编码 167aa 蛋白。该基因在其 N 端包含一个锌指基序，在其 C 端包含一个 YABBY 结构域。基于多序列比对，BoYAB1与水稻中的OsYAB1密切相关，序列同一性为88.76%。表达分析（RT-qPCR）和原位杂交结果表明，BoYAB1的优先积累在花蕾的雄蕊和心皮原基中。BoYAB1 在拟南芥中的异位表达导致开花晚，叶片窄而卷曲，卷曲指向背面。进一步的分析表明，观察到的表型差异与转基因植物中外源 BoYAB1 基因的表达水平有关。这些结果表明BoYAB1基因可能参与叶片发育和开花时间的调控。