Abstract The present study reports an electrochemical biosensor for the rapid and selective detection of L-arginine in leukemic blood samples. The biosensor was fabricated using a nanocomposite (PANI/MWCNTs/Fe3O4) film modified glassy carbon electrode (GCE). The partially purified arginine deiminsae (ADI) enzyme from P. putida was immobilized on the modified GCE by cross linking with gluteraldehyde. The characterization and validation of the developed biosensor was performed by various electrochemical techniques including cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry (CA). The limit of detection for biosensor was determined to be as low as 20 μM based on a signal-to-noise ratio equal to three while employing a detection period of 10 s. A linear response for L-arginine was observed across the concentration range from 100 to 1000 µM with an R2 value of 0.9987. The biosensor designed in the present study offers highly specific and sensitive analysis in normal and leukemic blood samples compared to previous reports in the literature. Therefore this device has excellent potential for nano-device fabrication for biomedical applications.

中文翻译：

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基于聚苯胺-多壁碳纳米管-磁铁矿纳米复合膜修饰玻碳电极电化学测定白血病血样中L-精氨酸

摘要 本研究报告了一种电化学生物传感器，用于快速和选择性检测白血病血样中的 L-精氨酸。使用纳米复合材料（PANI/MWCNTs/Fe3O4）膜修饰的玻璃碳电极（GCE）制造生物传感器。来自恶臭假单胞菌的部分纯化的精氨酸脱亚胺 (ADI) 酶通过与戊二醛交联固定在修饰的 GCE 上。所开发的生物传感器的表征和验证通过各种电化学技术进行，包括循环伏安法 (CV)、差分脉冲伏安法 (DPV) 和计时电流法 (CA)。基于等于 3 的信噪比，同时采用 10 秒的检测时间，生物传感器的检测限被确定为低至 20 μM。在 100 到 1000 µM 的浓度范围内观察到 L-精氨酸的线性响应，R2 值为 0.9987。与之前的文献报道相比，本研究中设计的生物传感器对正常和白血病血液样本进行了高度特异性和灵敏的分析。因此，该设备在用于生物医学应用的纳米设备制造方面具有出色的潜力。