Abstract Mesenchymal stem cells (MSCs) migrate to sites of inflammation and tumor formation in the body. In this way, MSCs become activated and release a plethora of growth factors and cytokines that are involved in the regulation and remodeling of the tumor microenvironment. The effect of MSCs on tumor cells is bipolar by nature and the data on this interaction is quite contradictory. The purpose of this work is to investigate the interaction between MSCs of different origin (bone marrow, adipose tissue) and PC-3 tumor cell line derived from prostate cancer in a long-term (9 days) cultivation. Our study assessed the proliferation, vitality and apoptosis of PC-3 tumor cells exposed to MSCs’ contact and products. As a result, we observed a significant growth inhibitory effect on PC-3 cells due to cell-to-cell contact with MSCs. Furthermore, tumor cells also showed contact-mediated increase in apoptosis levels. In addition, bone marrow-derived MSCs had a stronger effect on the PC-3 cell line compared to adipose tissue-derived MSCs.

中文翻译：

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间充质干细胞长期培养抑制前列腺癌细胞生长

摘要 间充质干细胞 (MSCs) 迁移到体内炎症和肿瘤形成的部位。通过这种方式，间充质干细胞被激活并释放大量参与肿瘤微环境调节和重塑的生长因子和细胞因子。MSCs 对肿瘤细胞的作用本质上是双极的，关于这种相互作用的数据是相当矛盾的。这项工作的目的是在长期（9 天）培养中研究不同来源（骨髓、脂肪组织）的 MSC 与源自前列腺癌的 PC-3 肿瘤细胞系之间的相互作用。我们的研究评估了暴露于 MSC 接触和产品的 PC-3 肿瘤细胞的增殖、活力和凋亡。结果，我们观察到由于与 MSC 的细胞间接触对 PC-3 细胞有显着的生长抑制作用。此外，肿瘤细胞也表现出接触介导的凋亡水平增加。此外，与脂肪组织来源的 MSCs 相比，骨髓来源的 MSCs 对 PC-3 细胞系的影响更强。