Abstract Plant invertases comprise a family of enzymes that catalyze the irreversible hydrolysis of sucrose into glucose and fructose, which plays a crucial role in carbohydrate partitioning and regulating plant growth and development. However, currently, there is little information on the roles of invertases in the development of pear trees. In this study, we performed genome-wide identification of a total of 23 invertases from the predicted genome data of the pear plant (Pyrus bretschneideri R.). Based on phylogenetic analysis, the pear invertases could be clustered into 13 acid invertase genes and 10 alkaline/neutral invertase genes. Chromosome location showed that tandem duplication and segmental duplication have played an important role in the expansion of acid and neutral/alkaline invertases in pear plants. Evolutionary analysis of invertases from pear and two model plant species (Arabidopsis thaliana and Oryza sativa) indicated that the evolutionary divergence of invertases occurred before the common ancestor of dicots and monocots. Tissue-specific expression analysis showed that the transcripts of all the identified invertase genes were detectable in various tissues. We hypothesize that the identified invertase genes all participate in regulating pear growth and development.

中文翻译：

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Pyrus bretschneideri 转化酶基因家族：鉴定、系统发育和表达模式

摘要 植物转化酶包括催化蔗糖不可逆水解为葡萄糖和果糖的酶家族，在碳水化合物分配和调节植物生长发育中起着至关重要的作用。然而，目前关于转化酶在梨树发育中的作用的信息很少。在这项研究中，我们从梨植物 (Pyrus bretschneideri R.) 的预测基因组数据中对总共 23 种转化酶进行了全基因组鉴定。根据系统发育分析，梨转化酶可分为13个酸性转化酶基因和10个碱性/中性转化酶基因。染色体定位表明串联重复和节段重复在梨植物酸性和中性/碱性转化酶的扩增中发挥了重要作用。梨和两种模式植物（拟南芥和水稻）转化酶的进化分析表明，转化酶的进化分歧发生在双子叶植物和单子叶植物的共同祖先之前。组织特异性表达分析表明，所有鉴定的转化酶基因的转录本都可在各种组织中检测到。我们假设所鉴定的转化酶基因都参与调控梨的生长发育。