Abstract Hip replacement is one of the most effective surgical procedures in medicine. Despite the fact that the clinical results of arthroplasty are usually excellent, some implants loosen with time and require revision. To help resolve this problem, research has focused on providing conditions that have a positive biological effect on bone building elements, resulting in a fast, seamless proliferation and differentiation of mesenchymal stem cells (MSC) into osteoblasts. That is why developing an in vitro model for studying MSC functions at the implant–tissue contact zone is essential. The aim of this study was to investigate the proliferation and differentiation capacity of human bone-marrow derived MSC in contact with medical implant-grade Ti-6Al-4V alloy and β-tricalcium phosphate (β-TCP). Human MSC were isolated from bone marrow aspirates obtained from the femoral canal during hip replacement surgeries and seeded on Ti-6Al-4V alloy squared specimens and β-TCP granules. The results indicate that MSC were able to effectively adhere to both biomaterial surfaces and, at the same time, retain their proliferative and osteogenic/adipogenic differentiation potential. We suggest that both Ti-6Al-4V alloy and β-TCP surfaces provide suitable conditions for the proliferation and differentiation of bone marrow MSC and can be used as in vitro models to explore MSC functions at the bone–implant interface.

中文翻译：

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TI-6AL-4V 合金和基于β-磷酸三钙的系统用于体外研究植入物-组织界面间充质干细胞功能

摘要 髋关节置换术是医学上最有效的外科手术之一。尽管关节置换术的临床结果通常非常好，但一些植入物会随着时间的推移而松动并需要修正。为了帮助解决这个问题，研究的重点是提供对骨骼构建元素具有积极生物学作用的条件，从而使间充质干细胞 (MSC) 快速、无缝地增殖和分化为成骨细胞。这就是为什么开发用于研究植入物-组织接触区 MSC 功能的体外模型至关重要的原因。本研究的目的是研究人骨髓源性 MSC 与医用植入级 Ti-6Al-4V 合金和 β-磷酸三钙 (β-TCP) 接触的增殖和分化能力。人类 MSC 从髋关节置换手术期间从股骨管获得的骨髓抽吸物中分离，并接种在 Ti-6Al-4V 合金方形标本和 β-TCP 颗粒上。结果表明，MSC 能够有效地粘附到两种生物材料表面，同时保留其增殖和成骨/成脂分化潜能。我们建议 Ti-6Al-4V 合金和 β-TCP 表面都为骨髓 MSC 的增殖和分化提供了合适的条件，并可用作体外模型来探索骨 - 植入物界面的 MSC 功能。保留其增殖和成骨/成脂分化潜能。我们建议 Ti-6Al-4V 合金和 β-TCP 表面都为骨髓 MSC 的增殖和分化提供了合适的条件，并可用作体外模型来探索骨 - 植入物界面的 MSC 功能。保留其增殖和成骨/成脂分化潜能。我们建议 Ti-6Al-4V 合金和 β-TCP 表面都为骨髓 MSC 的增殖和分化提供了合适的条件，并可用作体外模型来探索骨 - 植入物界面的 MSC 功能。