Abstract Rutin (Vitamin P1) is an antioxidant and anti-inflammatory flavonoid. This study examined the effect of rutin on the potential of cisplatin to cause oxidative and proinflammatory damage in blood serum, ureter, bladder and urethra in rats. Animals were divided into four groups: (i) healthy (HG), (ii) 50 mg/kg of rutin + 5 mg/kg of cisplatin (C + R50), (iii) 100 mg/kg of rutin + 5 mg/kg of cisplatin (C + R100) and (iv) only cisplatin (CG). Rutin and distilled water were applied once a day for eight days. Cisplatin was injected intraperitoneally at a dose of 5 mg/kg for eight days, once every two days. Finally, animals were sacrificed by high -dose anesthesia after blood samples were taken. Ureter, bladder and urethra tissues were removed and malondialdehyde (MDA), myeloperoxidase (MPO), total glutathione (tGSH), superoxide dismutase (SOD) interleukin beta (IL-1β) and tumor necrosis factor alpha (TNF-α) levels were measured. The results showed that the levels of MDA, MPO, IL-1β and TNF-α in the blood serum of the CG group increased significantly (p < 0.0001) and the tGSH and SOD levels decreased compared to the HG. Rutin administration reversed the effect as seen in C + R50 and C + R100 groups. However, there was no statistically significant difference in the levels of MDA, MPO, tGSH, SOD, IL-1β and TNF-α in ureter, bladder and urethra tissue (p > 0.05).This suggests that cisplatin produces oxidative stress in the blood serum but not in the ureter, bladder and urethra tissues. It was observed that rutin prevented the cisplatin-related oxidative and proinflammatory damage in the blood serum.

中文翻译：

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芦丁对顺铂诱导大鼠血清、输尿管、膀胱和尿道氧化和促炎性损伤的影响

摘要 芦丁（Vitamin P1）是一种抗氧化和抗炎的黄酮类化合物。本研究检查了芦丁对顺铂在大鼠血清、输尿管、膀胱和尿道中引起氧化和促炎性损伤的可能性的影响。动物分为四组：(i) 健康 (HG)，(ii) 50 mg/kg 芦丁 + 5 mg/kg 顺铂 (C + R50)，(iii) 100 mg/kg 芦丁 + 5 mg/ kg 顺铂 (C + R100) 和 (iv) 仅顺铂 (CG)。每天使用一次芦丁和蒸馏水，持续八天。顺铂以5mg/kg的剂量腹膜内注射8天，每两天一次。最后，取血样后通过高剂量麻醉处死动物。去除输尿管、膀胱和尿道组织并检测丙二醛 (MDA)、髓过氧化物酶 (MPO)、总谷胱甘肽 (tGSH)、测量了超氧化物歧化酶（SOD）白细胞介素β（IL-1β）和肿瘤坏死因子α（TNF-α）水平。结果显示，与HG相比，CG组血清中MDA、MPO、IL-1β和TNF-α水平显着升高（p < 0.0001），tGSH和SOD水平降低。如在 C + R50 和 C + R100 组中所见，芦丁给药逆转了效果。但输尿管、膀胱和尿道组织中MDA、MPO、tGSH、SOD、IL-1β和TNF-α水平差异无统计学意义（p > 0.05）。这表明顺铂在血液中产生氧化应激血清，但不在输尿管、膀胱和尿道组织中。据观察，芦丁可防止血清中与顺铂相关的氧化和促炎性损伤。