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Characterization of LcGAPC and its transcriptional response to salt and alkali stress in two ecotypes of Leymus chinensis (Trin.) Tzvelev
Biotechnology & Biotechnological Equipment ( IF 1.4 ) Pub Date : 2020-01-01 , DOI: 10.1080/13102818.2020.1719020 Qian Lu 1 , Xue Meng 1 , Fanghui Yang 1 , Xin Liu 1 , Jizhe Cui 1
Biotechnology & Biotechnological Equipment ( IF 1.4 ) Pub Date : 2020-01-01 , DOI: 10.1080/13102818.2020.1719020 Qian Lu 1 , Xue Meng 1 , Fanghui Yang 1 , Xin Liu 1 , Jizhe Cui 1
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Abstract Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a housekeeping protein which plays various roles in non-metabolic processes in addition to its role in glucose catabolism. There are several GAPDH isoforms in plant cells. In this study, we cloned the full-length cDNA encoding the isoform GAPC in two ecotypes of Leymus chinensis (Trin.) Tzvelev, gray–green type and yellow–green type. The LcGAPC sequence includes an open reading frame (ORF) of 1014 bp encoding 337 amino acids. The predicted molecular weight is 36.5 kDa, and the pI is 6.19. The mRNA expression of LcGAPC decreased at 24 h and then increased significantly after 7 d after 400 mmol/L NaCl stress. At 200 mmol/L mixed alkali stress (NaHCO3:Na2CO3=9:1), the LcGAPC expression level gradually increased as time increased in the two ecotypes of L. chinensis. The results suggested that LcGAPC is a stress-inducible gene that might play a role in the salt and alkali stress response. This study provided a basis to further study the mechanism of expression characteristics under salt and alkali stress conditions of L. chinensis.
中文翻译:
LcGAPC 的特征及其在两种生态型羊草 (Trin.) Tzvelev 中对盐碱胁迫的转录响应
摘要 3-磷酸甘油醛脱氢酶(GAPDH)是一种管家蛋白,除了在葡萄糖分解代谢中发挥作用外,在非代谢过程中也发挥着多种作用。植物细胞中有几种 GAPDH 同种型。在这项研究中,我们克隆了编码同种型 GAPC 的全长 cDNA 在两种生态型羊草 (Trin.) Tzvelev,灰绿色型和黄绿色型。LcGAPC 序列包括一个 1014 bp 的开放阅读框 (ORF),编码 337 个氨基酸。预测分子量为 36.5 kDa,pI 为 6.19。400 mmol/L NaCl胁迫后,LcGAPC mRNA表达在24 h下降,7 d后显着升高。在200 mmol/L混合碱胁迫下(NaHCO3:Na2CO3=9:1),LcGAPC表达水平随着时间的增加,在L. chinensis 2个生态型中逐渐升高。结果表明,LcGAPC 是一种胁迫诱导基因,可能在盐碱胁迫反应中发挥作用。本研究为进一步研究盐碱胁迫条件下紫菜表达特性的机制提供了依据。
更新日期:2020-01-01
中文翻译:
LcGAPC 的特征及其在两种生态型羊草 (Trin.) Tzvelev 中对盐碱胁迫的转录响应
摘要 3-磷酸甘油醛脱氢酶(GAPDH)是一种管家蛋白,除了在葡萄糖分解代谢中发挥作用外,在非代谢过程中也发挥着多种作用。植物细胞中有几种 GAPDH 同种型。在这项研究中,我们克隆了编码同种型 GAPC 的全长 cDNA 在两种生态型羊草 (Trin.) Tzvelev,灰绿色型和黄绿色型。LcGAPC 序列包括一个 1014 bp 的开放阅读框 (ORF),编码 337 个氨基酸。预测分子量为 36.5 kDa,pI 为 6.19。400 mmol/L NaCl胁迫后,LcGAPC mRNA表达在24 h下降,7 d后显着升高。在200 mmol/L混合碱胁迫下(NaHCO3:Na2CO3=9:1),LcGAPC表达水平随着时间的增加,在L. chinensis 2个生态型中逐渐升高。结果表明,LcGAPC 是一种胁迫诱导基因,可能在盐碱胁迫反应中发挥作用。本研究为进一步研究盐碱胁迫条件下紫菜表达特性的机制提供了依据。