Abstract

Waterlogging is a global abiotic stress that seriously restricts the growth and yield of wheat. DNA methylation is the most common epigenetic modification and plays an important role in plant responses to adverse conditions. In this study, the methylation-sensitive amplification polymorphism (MSAP) sequencing approach was used to detect the methylation levels and pattern changes under hypoxia stress in different waterlogging tolerance wheat (Triticum aestivum L.) genotypes. Methyltransferase gene expression and protein levels were higher in the tolerant wheat genotype. Although demethylation occurred predominantly in both wheat genotypes, waterlogging-related genes, such as ERF1, ACC1, and CKX2.3, were significantly upregulated only in the tolerant genotype by demethylation, whereas other genes, such as RGA2, were significantly downregulated only in the sensitive genotype. N⁶-Methyladenosine (m⁶A) content decreased significantly in the leaves of the sensitive genotype but remained unchanged in the tolerant genotype. Our results showed that both DNA and RNA methylations play a regulatory role in wheat response to waterlogging stress.

中文翻译：

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甲基转移酶调控小麦对低氧胁迫响应的甲基化变化

摘要

涝渍是一种全球性的非生物胁迫，严重限制了小麦的生长和产量。DNA甲基化是最常见的表观遗传修饰，并且在植物对不利条件的反应中起重要作用。在这项研究中，甲基化敏感扩增多态性（MSAP）测序方法用于检测在不同耐涝小麦（Triticum aestivum L.）基因型低氧胁迫下的甲基化水平和模式变化。耐性小麦基因型的甲基转移酶基因表达和蛋白质水平较高。尽管脱甲基化主要发生在两种小麦基因型中，但与涝渍有关的基因（例如ERF1，ACC1和CKX2.3），仅通过脱甲基化仅在耐受基因型中显着上调，而其他基因，例如RGA2，仅在敏感基因型中显着下调。在敏感基因型的叶片中，N ^6-甲基腺苷（m ⁶ A）含量显着下降，但在耐受基因型中则保持不变。我们的结果表明，DNA和RNA甲基化在小麦对涝渍胁迫的响应中起调节作用。