The entomopathogenic fungi are safe and ecofriendly biocontrol agents which can be applied against stored product pests. In this study, potentials of some Iranian isolates of Lecanicillium lecanii (PAL6, PAL7 and PAL8) and Lecanicillium muscarium to control Tribolium castaneum (AGM5) were investigated under laboratory conditions. For this purpose, the LC₅₀ and LT₅₀ values of each isolates to adults of the beetle were estimated. Results showed that all of the isolates were virulent to the pest. The estimated LC₅₀ values were ranged from 2.8 × 10⁶ to 6.13 × 10²² (conidia/mL). The lowest and the highest LC₅₀ values were estimated for L. lecanii (PAL7) and L. mucarium (isolate AGM5), respectively. The LT₅₀ values varied from 4.99 to 13.65 days. The LT₅₀ values were decreased by increasing conidial concentrations of each isolate. Moreover, at each conidial concentration, the LT₅₀ values for L. lecanii isolate PAL8 were significantly lower than other isolates. Totally, among the isolates, L. lecanii isolate PAL7 (at concentration 10⁷ conidia/mL) is the most promising isolate. The laboratory study is preliminary, and further experiments under field condition are required before any recommendations.

中文翻译：

---

Lecanicillium lecanii的某些分离物对控制Tribolium castaneum（Col.，Tenebrionidae）的功效

昆虫病原性真菌是安全，环保的生物防治剂，可用于对抗储存的产品害虫。在这项研究中，在实验室条件下研究了伊朗分离出的Lecanicillium lecanii（PAL6，PAL7和PAL8）和Lecanicillium muscarium的潜力，以控制cast藜（Tribolium castaneum）（AGM5）。为此，估计了甲虫成虫的每个分离株的LC ₅₀和LT ₅₀值。结果表明，所有分离株均对害虫具有毒性。估计的LC ₅₀值在2.8×10 ⁶至6.13×10 ²²（分生孢子/ mL）的范围内。最低和最高LC ₅₀分别对lecanii L.（PAL7）和M. mucarium（分离的AGM5）进行估计。LT ₅₀值从4.99天到13.65天不等。通过增加每个分离株的分生孢子浓度降低LT ₅₀值。此外，在每个分生孢子浓度时，LT ₅₀为值L.枝菌三级分离PAL8比其它分离显著低。完全，分离株，其中L.枝菌三级分离PAL7（在浓度为10 ⁷个分生孢子/毫升）是最有前途的分离物。实验室研究是初步的，在提出任何建议之前，需要在野外条件下进行进一步的实验。