miRNAs are important regulators of plant gene expression. There are few studies on the regulation of miRNAs in Lonicera edulis. We used high-throughput sequencing technology to analyse miRNAs in L. edulis, aiming to identify miRNAs and elucidate their function in L. edulis. In the present study, we employed the high-throughput sequencing technology to profile miRNAs in L. edulis. A total of 51,819,072 small RNA tags with sizes ranging from 18 to 30 nt were obtained, indicating that L. edulis have a large and diverse small RNA population. Bioinformatic analysis identified 507 mature miRNAs, and 16 predicted novel miRNAs that are likely to be unique to L. edulis. Three miRNAs related to anthocyanin biosynthesis were locked by gene ontology (GO) analysis and target gene analysis. The selected three miRNAs are relatively high in the expression of L. edulis. Some of the previous studies have studied these types of miRNAs involved in the anthocyanin metabolism pathway in fruits. Among them, expression profiles of three conserved miRNAs were validated by stem loop qRT-PCR. Further, the potential target genes of conserved and novel miRNAs were predicted and subjected to GO annotation. Enrichment analysis of the GO-represented biological processes and molecular functions revealed that these target genes were potentially involved in a wide range of metabolic pathways and developmental processes. In particular, different families of miRNAs can directly or indirectly regulate anthocyanin biosynthesis. In recent years, the research on miRNAs has become more and more clear, but the research on miRNAs involved in the regulation of anthocyanin synthesis of L. edulis is still lagging. This study provides a useful resource for further elucidation of the functional roles of miRNAs during fruit development and ripening.

中文翻译：

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高通量测序鉴定金银花中与花青素生物合成相关的微小RNA和总微小RNA

miRNA 是植物基因表达的重要调控因子。金银花中miRNAs调控的研究较少。我们采用高通量测序技术对青蒿中的miRNA进行分析，旨在鉴定miRNA并阐明其在青蒿中的功能。在本研究中，我们采用高通量测序技术来分析 L. edulis 中的 miRNA。总共获得了 51,819,072 个大小从 18 到 30 nt 的小 RNA 标签，表明 L. edulis 具有大量且多样化的小 RNA 种群。生物信息学分析确定了 507 个成熟的 miRNA，16 个预测的新 miRNA 可能是 L. edulis 独有的。通过基因本体（GO）分析和靶基因分析锁定了与花青素生物合成相关的三个miRNA。所选出的3个miRNA在L的表达上都比较高。杜利斯。之前的一些研究已经研究了参与水果中花青素代谢途径的这些类型的miRNA。其中，通过茎环 qRT-PCR 验证了三个保守 miRNA 的表达谱。此外，预测了保守和新型 miRNA 的潜在靶基因并进行了 GO 注释。GO 代表的生物过程和分子功能的富集分析表明，这些靶基因可能参与广泛的代谢途径和发育过程。特别是，不同家族的 miRNA 可以直接或间接调节花青素的生物合成。近年来，关于miRNAs的研究越来越清晰，但对参与调控香菇花青素合成的miRNAs的研究还很滞后。