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Molecular phylogeny of Monomorium pharaonis (Hymenoptera: Formicidae) based on rRNA sequences of mitochondrial gene
Journal of Genetics ( IF 1.5 ) Pub Date : 2020-04-10 , DOI: 10.1007/s12041-020-1186-y
Reem Alajmi , Rania Haddadi , Rewaida Abdel-Gaber , Mohammed Alkuriji

Many studies on forensic entomology have focussed on Diptera and lack the Hymenopteras population. Nonetheless, hymenopterans are part of the entomofaunal colonization of a corpse. Morphologically, it is difficult to identify and distinguish between them. In this study, using mitochondrial DNA knowledge, the molecular analysis was performed to classify the recovered species of hymenoptera collected from rabbit carcass, quickly and accurately. A molecular identification method with a 251-bp fragment of the 16S ribosomal gene RNA (16S rRNA) from a single ant species was evaluated. The maximum likelihood method analysis has recovered a generally well-supported phylogeny, with most taxa and species groups currently being recognized as monophyletic. The aculeate consists of some Hymenoptera’s best known. Their sister group has traditionally been considered in Ichneumonoidea. In addition, Trigonaloidea was found as the aculeates’ sister group and Crabronidae in Apoidea forming the Formicidae’s sister group. These results will play an important role in the implementation of the Saudi database forensically relevant ants.

中文翻译:

基于线粒体基因rRNA序列的Monmorium pharaonis(膜翅目:蚁科)的分子系统发育

许多关于法医昆虫学的研究都集中在双翅目上,而缺乏膜翅目种群。尽管如此,膜翅目昆虫是尸体昆虫群落的一部分。在形态上,很难识别和区分它们。本研究利用线粒体DNA知识,对从兔尸体中采集的膜翅目回收物种进行分子分析,快速准确地进行分类。评估了使用来自单个蚂蚁物种的 16S 核糖体基因 RNA (16S rRNA) 的 251 bp 片段的分子鉴定方法。最大似然法分析恢复了普遍得到充分支持的系统发育,大多数分类群和物种群目前被认为是单系的。有刺包括一些膜翅目最著名的。他们的姊妹组传统上被认为是在 Ichneumonoidea 中。此外,在Apoidea中发现Trigonaloidea作为有刺目的姐妹群和Crabronidae形成蚁科的姐妹群。这些结果将在沙特数据库法医相关蚂蚁的实施中发挥重要作用。
更新日期:2020-04-10
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