Using comet assay DNA damage in the cells of Hypothenemus hampei adults was assessed following irradiation from a 60Co source at gamma radiation doses 10, 20, 40, 80, 160, 320, 640, 1600 and 3200 Gy. The survival of exposed adults at different interval of time was assessed for the same doses of radiation. Radiation-induced DNA damage measured by increased strand breaks at higher doses was significantly different from intact cells found in control, where the increase in damage was dose-dependent. The survival of the exposed adults was dose-dependent. Doses of 1600 and 3200 Gy resulted in 100% mortality within 15 days of irradiation. The absence of significant radiation induced changes in DNA damage and survival of adults at lower doses indicates greater tolerance to radiation. The study provides useful information about the radiation sensitivity of H. hampei to develop an effective method for the control of the borer in harvested coffee.

中文翻译：

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咖啡浆果蛀虫 Hypothenemus hampei Ferrari（鞘翅目： Curculionidae）成虫的伽马辐射引起的 DNA 损伤

使用彗星测定法在伽马辐射剂量为 10、20、40、80、160、320、640、1600 和 3200 Gy 的 60Co 源照射后，评估了 Hypothenemus hampei 成虫细胞中的 DNA 损伤。对于相同剂量的辐射，评估了不同时间间隔暴露的成年人的存活率。通过在较高剂量下增加的链断裂来测量的辐射诱导的 DNA 损伤与在对照中发现的完整细胞显着不同，其中损伤的增加是剂量依赖性的。暴露的成年人的存活率是剂量依赖性的。1600 和 3200 Gy 的剂量在照射后 15 天内导致 100% 的死亡率。在较低剂量下，成年人的 DNA 损伤和存活率没有显着的辐射引起的变化，表明对辐射的耐受性更高。