BACKGROUND Sweet potato is easily propagated by cuttings. But the molecular biological mechanism of adventitious root formation are not yet clear. OBJECTIVE To understand the molecular mechanisms of adventitious root formation from stem cuttings in sweet potato. METHODS RNA-seq analysis was performed using un-rooted stem (0 day) and rooted stem (3 days). Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, comparison with Arabidopsis transcription factors (TFs) of DEGs were conducted to investigate the characteristics of genes and TFs involved in root formation. In addition, qRT-PCR analysis using roots at 0, 3, 6, 9, and 12 days after planting was performed to confirm RNA-seq reliability and related genes expression. RESULTS 42,459 representative transcripts and 2092 DEGs were obtained through the RNA-seq analysis. The DEGs indicated the GO terms related to the single-organism metabolic process and cell periphery, and involved in the biosynthesis of secondary metabolites, and phenylpropanoid biosynthesis in KEGG pathways. The comparison with Arabidopsis thaliana TF database showed that 3 TFs (WRKY, NAC, bHLH) involved in root formation of sweet potato. qRT-PCR analysis, which was conducted to confirm the reliability of RNA-seq analysis, indicated that some metabolisms including oxidative stress and wounding, transport, hormone may be involved in adventitious root formation. CONCLUSIONS The detected genes related to secondary metabolism, some hormone (auxin, gibberellin), transports, etc. and 3 TFs (WRKY, NAC, bHLH) may have functions in adventitious roots formation. This results provide valuable resources for future research on the adventitious root formation of sweet potato.

中文翻译：

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通过对甘薯（Ipomoea batatas（L.）Lam。）根形成过程中的转录组进行综合分析来获得功能基因组学。

背景技术甘薯容易通过插条繁殖。但是不定根形成的分子生物学机制尚不清楚。目的了解甘薯茎cutting不定根形成的分子机制。方法采用无根茎（0天）和无根茎（3天）进行RNA-seq分析。进行了基因本体论（GO）富集分析，京都基因与基因组百科全书（KEGG）途径，与DEGs的拟南芥转录因子（TFs）进行比较，以研究参与根形成的基因和TFs的特征。另外，在种植后0、3、6、9和12天使用根进行qRT-PCR分析，以确认RNA-seq的可靠性和相关基因的表达。结果42 通过RNA-seq分析获得了459个代表性转录物和2092个DEG。DEGs指示与单生物代谢过程和细胞外围有关的GO术语，并参与KEGG途径的次生代谢产物的生物合成和苯丙烷的生物合成。与拟南芥TF数据库的比较表明，3个TF（WRKY，NAC，bHLH）参与了甘薯的根形成。进行qRT-PCR分析以确认RNA-seq分析的可靠性，结果表明不定根的形成可能涉及某些代谢，包括氧化应激和创伤，转运，激素。结论检测到的与次生代谢，一些激素（生长素，赤霉素），转运等相关的基因和3个TF（WRKY，NAC，bHLH）可能具有不定根形成的功能。