As the energizing and therapeutic effects of infusions of mate (Ilex paraguariensis A.St.-Hil) leaves are increasingly recognized, rising global consumption is also increasing the demand for plantlets with high genetic and morphological qualities. Mini-cuttings are an alternative for clonal plantlets; however, more details are needed on factors that affect the success of this technique. Here, toward maximizing production, we evaluated adventitious rooting competence of mini-cuttings of different sizes and the morphophysiological quality of the resultant plantlets. Small (one bud; length up to 2.5 cm), medium (multiple buds; length 2.6–5.0 cm), and large (multiple buds; length 5.1–10.0 cm) mini-cuttings were planted in a mixture of commercial substrate, vermiculite, and coarse sand (1:1:1 v:v:v), grown in a humidity chamber, then evaluated after 45, 60, 75, and 90 days for survival, percentage of callus and shoot formation, number and length of shoots, percentage of rooted cuttings, and number and length of roots. Any rooted mini-cuttings were then cultivated in commercial substrate and subsurface soil (2:1 v:v) and evaluated after 30, 60, 90 and 120 days of cultivation for morphophysiology of the plantlets, percentage of survival, number of leaves, shoot height, stem diameter, and ratio of shoot height to stem diameter. After 120 days, the plantlets were evaluated for the ratio of shoot and root dry mass, total length, surface area, total volume, and mean diameter of roots and Dickson quality index. Adventitious rooting of the mini-cuttings and the morphophysiological quality of the plantlets were not influenced by the size of the vegetative propagule. Therefore, single-bud mini-cuttings have similar adventitious rooting competence, and result in plantlets with adequate morphophysiological quality, based upon leaf development, shoot height, stem diameter and length, and surface area, volume and diameter of roots. Single-bud mini-cuttings up to 2.5 cm long can thus be used to maximize production of mate plantlets by mini-cuttings.

作为伴侣的输注的通电和治疗效果（冬青巴拉圭越来越多地认识到A.St.-Hil）叶片，全球消费量的增长也增加了对具有高遗传和形态学品质的小苗的需求。小型插条是无性系苗的替代品；但是，关于影响该技术成功的因素，还需要更多细节。在这里，为了使产量最大化，我们评估了不同大小的小型插条的不定根生根能力以及所得幼苗的形态生理质量。将小块（一个芽；长至2.5厘米），中等（多个芽；长2.6–5.0厘米）和大（多个芽；长5.1–10.0厘米）微型切块种植在商业基质，ver石，和粗砂（1：1：1 v：v：v），在湿度箱中生长，然后在45、60、75和90天后评估存活率，愈伤组织和芽形成的百分比，芽的数量和长度，生根的插条的百分比以及根的数量和长度。然后将任何生根的小块插穗种植在商品基质和地下土壤（2：1 v：v）中，并在培养30、60、90和120天后评估幼苗的形态生理，存活率，叶数，芽高度，茎直径以及枝条高度与茎直径的比率。120天后，评估小苗的茎和根干重之比，总长度，表面积，总体积，根的平均直径和Dickson质量指数。小插穗不定根和小苗的形态生理质量不受营养繁殖体大小的影响。因此，单芽小型插穗具有类似的不定根生根能力，根据叶片的发育，枝条的高度，茎的直径和长度以及根的表面积，体积和直径，可以使小植株具有足够的形态生理品质。因此，最长可达2.5厘米的单芽微型插条可用于通过微型插条最大化交配小植株的产量。