Because overexpression of Vitreoscilla hemoglobin gene (Vgb) gene in plants can enhance tolerance to waterlogging, here Vgb was inserted into Populus alba × glandulosa to investigate its expression and effects on growth and physiological responses to waterlogging stress in the transgenic poplars. Southern blotting and RT-PCR analysis of Vgb-transgenic P. alba × glandulosa showed that the Vgb gene was integrated into the genome of the V13-81 and V13-85 transgenic lines and expressed. In greenhouse waterlogging stress tests, mortality of the transgenic poplar was significant lower than that of nontransgenic plants with increasing treatment time from 2 to 22 days. The transgenic plants had higher chlorophyll content and less chloroplast damage than in the control plants. Additionally, starch accumulation increased, and growth was enhanced in the transgenic plants, suggesting that the Vgb-expressing lines had improved energy reserves. Field trials of the transgenic poplar suggested that Vgb expression promotes growth and influences wood quality. Taken together, our results suggest that the expression of Vgb can increase the accumulation of chlorophyll and starch in the transgenic poplar, improve its ability to endure flooding, and improve growth and wood quality of the transgenic plants.

因为过度表达透明颤菌血红蛋白基因（Vgb的植物）基因可以增强耐受涝，这里Vgb的插入银白杨 × 腺其上生长和生理反应的表达和作用进行调查，以在转基因杨树淹水应力。Vgb-转基因毕赤酵母（P. alba  ×  glandulosa）的Southern印迹和RT-PCR分析表明，Vgb该基因被整合到V13-81和V13-85转基因株的基因组中并表达。在温室涝渍胁迫试验中，随着处理时间从2天增加到22天，转基因杨树的死亡率显着低于非转基因植物。与对照植物相比，转基因植物具有更高的叶绿素含量和更少的叶绿体破坏。另外，在转基因植物中淀粉积累增加，并且生长增强，表明表达Vgb的品系具有改善的能量储备。转基因杨树的田间试验表明，Vgb表达可促进生长并影响木材质量。综上所述，我们的结果表明Vgb的表达 可以增加转基因杨树中叶绿素和淀粉的积累，提高其抗洪能力，并改善转基因植物的生长和木材品质。