Aenasius arizonensis (Hymenoptera: Encyrtidae) a nymphal, solitary endoparasitoid is a potent biocontrol agent of cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae). Female wasp injects a blend of secretions in its host during parasitization which alters the host physiology. Insecticidal potential of crude venom of A. arizonensis was evaluated along with its chromatographic fractions by microinjections bioassays in its natural host insect P. solenopsis at 3rd nymphal instars and adult. Host insects were treated with two different volumes of crude venom (0.3 and 0.5 µL) and RP-HPLC fractions of venom, while host insects with saline injections were used as control. Biological activity of the crude venom was also evaluated after heat and protease treatment. Maximum mortality (84.0 ± 2.91) of the host insects was observed at highest dose rate (0.5 µL) of the crude venom; while lowest mortality (71.0 ± 2.31) was recorded at lowest dose (0.3 µL) of venom applied on 3rd instar life stage. On the other hand, significant difference in mortality of four peaks of RP-HPLC venom fractions were recorded against 3rd instar larvae and adult when microinjections containing crude venom and fractionated venom. Our data confirms that crude venom of the wasp induces significant mortality against its host P. solenopsis. Whereas, no significant difference in mortality was observed for heat and protease treated venom, suggesting that bioactive component of the venom secretions of the wasp are proteins in nature, which lost their biological activity upon heat and protease treatments.

若虫，孤立的内寄生物Aenasius arizonensis（膜翅目：Encyrtidae）是棉花粉虱Phenacoccus solenopsis Tinsley（半翅目：Pseudococcidae）的有效生物防治剂。雌性黄蜂在寄生过程中会在宿主体内注入分泌物混合物，从而改变宿主的生理机能。通过显微注射生物测定法在其天然宿主昆虫P. solenopsis中评估了A. arizonensis粗毒的杀虫潜力及其色谱级分在三龄若虫和成虫。用两种不同体积的粗毒液（0.3和0.5 µL）和RP-HPLC级毒液处理寄主昆虫，同时将注射盐水的寄主昆虫用作对照。在加热和蛋白酶处理之后，还评估了粗毒液的生物活性。在最高剂量率（0.5 µL）的粗毒液中观察到宿主昆虫的最大死亡率（84.0±2.91）。而在第三龄期的生命中，以最低剂量（0.3 µL）的毒液记录的最低死亡率（71.0±2.31）。另一方面，当显微注射含有粗毒液和分馏毒液的三龄幼虫和成虫时，RP-HPLC毒液组分的四个峰的死亡率有显着差异。我们的数据证实，黄蜂的毒液会诱导其寄主的大量死亡斑节对虾。然而，加热和蛋白酶处理的毒液的死亡率没有观察到显着差异，表明黄蜂毒液分泌物中的生物活性成分是自然界中的蛋白质，在加热和蛋白酶处理后其生物活性丧失。