当前位置: X-MOL 学术Conserv. Genet. Resour. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A novel environmental DNA detection approach for the wading birds Platalea leucorodia , Recurvirostra avosetta and Tringa totanus
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2020-04-02 , DOI: 10.1007/s12686-020-01143-x
Robin Schütz , Ralph Tollrian , Maximilian Schweinsberg

Wading birds play an important role in coastal and wetland ecosystems. As a result of anthropogenic disturbances, numbers of wading birds have declined over the past years, and therefore, the monitoring of bird species is crucial to preserve their habitats and counteract this. Due to known limitations of conventional monitoring, molecular approaches are increasingly becoming complementary methods. Thus, we evaluated the potential of an environmental DNA (eDNA) monitoring approach for wading birds. We developed three species-specific primer sets for wading birds targeting the COI region. After examining the primers in silico and on extracted bird DNA, the primers were used to successfully amplify eDNA from water samples.



中文翻译:

一种涉水鸟类白斑病,白纹病和白斑病的新型环境DNA检测方法

涉禽在沿海和湿地生态系统中起着重要作用。由于人为干扰,过去几年涉水鸟的数量有所减少,因此,对鸟类的监视对于保护其栖息地并加以抵制至关重要。由于常规监测的已知局限性,分子方法越来越成为补充方法。因此,我们评估了涉水鸟类的环境DNA(eDNA)监测方法的潜力。我们针对针对COI区域的涉禽开发了三种特定于物种的引物组。在计算机上和提取的鸟类DNA上检查了引物后,将这些引物用于成功扩增水样中的eDNA。

更新日期:2020-04-02
down
wechat
bug