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Molecular sexing is a viable alternative to probing for determining sex in the imperiled Louisiana Pine Snake ( Pituophis ruthveni )
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2020-03-29 , DOI: 10.1007/s12686-020-01145-9
Aron D. Katz , Sarah Pearce , Christopher Melder , Jinelle H. Sperry , Mark A. Davis

Determining sex is critical for demographic monitoring of endangered species, but this task can be particularly challenging in reptiles that are sexually monomorphic. Conventional sexing methods are invasive, often inaccurate, can cause injury, and, for sexually immature individuals, equivocal. Molecular approaches have been established as viable alternatives but have not been compared with conventional methods. Here we compare the performance of cloacal probing with a molecular genetic approach to determine sex in Louisiana Pine Snakes. This highly endangered species is rare, cryptic, and largely fossorial, presenting particular difficulties for assessing their population status. Molecular assays produced unambiguous sex assignments for all individuals, whereas probing recovered an incorrect or unidentified sex 28% of the time. These results reveal that molecular sexing outperforms probing for determining sex in Louisiana Pine Snakes, suggesting management programs for other snakes of conservation concern could benefit by incorporating similar genetic approaches.



中文翻译:

分子性别鉴定是代替探测确定绝种的路易斯安那松蛇(Pituophis rut​​hveni)性别的可行选择。

确定性别对于进行濒危物种的人口统计监测至关重要,但是对于具有单性的爬行动物而言,这项任务尤其具有挑战性。传统的性爱方法是侵入性的,通常是不准确的,会造成伤害,并且对于性未成熟的人来说是模棱两可的。分子方法已被确定为可行的替代方法,但尚未与常规方法进行比较。在这里,我们比较了泄殖腔探测的性能与分子遗传学方法以确定路易斯安那州松蛇的性别。这种高度濒临灭绝的物种是稀有,隐秘的,并且大部分为窝,对评估其种群状况特别困难。分子测定法为所有个体产生了明确的性别分配,而探测则有28%的时间恢复了不正确或不确定的性别。

更新日期:2020-03-29
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