Cerastium arcticum is one of the few flowering plants that thrives in the Arctic, suggesting that it possesses mechanisms for overcoming this extreme environment. To verify the functionality of C. arcticum dehydrin (CaDHN), known to play a protective role during cellular dehydration, the gene was introduced to Arabidopsis and Escherichia coli. Transgenic plants expressing CaDHN had significantly greater fresh weight and relative water content than wild-type plants under 15% PEG treatments, representing enhanced tolerance to drought. Under chilling conditions, transgenic plants remained vivid green, containing about 1.35-fold higher levels of chlorophyll than wild-type plants, and transgenic seeds germinated 2–3 days earlier with approximately threefold higher germination rates than wild-type seeds. Furthermore, oxidative stress under chilling conditions was 30% lower in transgenic plants than in wild-type plants. Transgenic plants also showed improved tolerance to various stresses, such as cold shock and salinity. In addition, transformed E. coli expressing CaDHN also showed enhanced tolerance to stress conditions, which suggests that CaDHN is conserved across taxa to provide tolerance to stress. These results indicate that CaDHN plays an important role in conferring tolerance to oxidative stress.

中文翻译：

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北极植物Cerastium arcticum的脱水蛋白基因Ca DHN增加了拟南芥对多种胁迫的耐受性

cercerium arcticum是在北极繁衍生息的为数不多的开花植物之一，表明它具有克服这种极端环境的机制。要验证的功能C. arcticum脱水（钙DHN），已知发挥细胞脱水过程中的保护作用，该基因被引入到拟南芥和大肠杆菌。表达Ca的转基因植物在15％PEG处理下，DHN的鲜重和相对水分含量明显高于野生型植物，表明对干旱的耐受性增强。在低温条件下，转基因植物保持鲜绿色，叶绿素含量比野生型植物高约1.35倍，转基因种子在2-3天前发芽，发芽率比野生型种子高约三倍。此外，转基因植物在寒冷条件下的氧化应激比野生型植物低30％。转基因植物还显示出对各种胁迫（例如冷休克和盐度）的耐受性提高。此外，表达Ca DHN的转化大肠杆菌还显示出对胁迫条件的增强耐受性，这表明CaDHN在整个分类单元中都是保守的，以提供对压力的耐受性。这些结果表明Ca DHN在赋予对氧化应激的耐受性中起重要作用。