To effectively discriminate changes in blood samples, RBCs aggregation and blood viscosity should be evaluated independently and simultaneously. In this study, by setting two syringe pumps for delivering two fluids (blood sample and reference fluid) at stepwise varying flow rates, RBCs aggregation (AI) and viscosity (µ_Blood) are sequentially measured by quantifying image intensity of blood flows (<I>) and interface between blood sample and reference fluid (α_Blood) in microfluidic channels. The (<I>) for measuring AI was obtained at lower shear rate (γ̇ < 91.7 s^-1). Additionally, α_Blood for measuring µ_Blood was obtained at higher shear rates (γ̇ < 91.7 s^-1). As a demonstration, the proposed method is employed to measure AI and µ_Blood for various blood samples composed of different concentrations of dextran solution or different degrees in RBCs deformability. After then, the method is employed to measure AI and µ_Blood of blood samples with respect to storage time of 25 days. As a result, RBCs aggregation and blood viscosity varied continuously up to 15 days of storage time. In conclusion, this method can be used effectively to detect changes in blood samples by measuring µ_mood and AI of blood samples simultaneously.

中文翻译：

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在逐步变化的剪切速率下通过测量RBC聚集和血液粘度，基于微流体的血液有效监测

为了有效地区分血液样本中的变化，应独立并同时评估RBC的聚集和血液粘度。在这项研究中，通过调节两个注射器在逐步输送两种流体（血液样品和参考流体）变化的流速泵，红细胞聚集（AI）和粘度（μ_血液）通过定量的血液的图像强度被顺序地测量流（<我>）以及微流体通道中的血液样本和参考液（α_血液）之间的界面。以较低的剪切速率（γ̇<91.7s ^-1）获得用于测量AI的（< I > ）。此外，α_血用于测量µ的_血液是在较高的剪切速率下（γ̇<91.7 s ^-1）获得的。作为演示，该提议的方法用于测量由不同浓度的葡聚糖溶液或不同程度的RBC变形性组成的各种血液样本的AI和µ _Blood。此后，该方法用于相对于25天的存储时间测量_血样的AI和µ _Blood。结果，RBC的聚集和血液粘度在长达15天的存储时间中连续变化。总之，该方法可通过测量µ_情绪和噪声来有效地检测血液样本中的变化。血液样本的AI同时进行。