As a class of AP2 family transcription factors, C-repeat binding factor (CBF) gene family plays an important role in resisting low-temperature stress and improving cold tolerance of plants. Rosa chinensis is an important horticultural and ornamental plant, but little is known about the molecular characteristics of CBF genes in R. chinensis. In our study, six RcCBFs members were identified at R. chinensis whole genome, including one member RcCBF1 on Chromosome 1 and other five members RcCBF2-6 on Chromosome 7. The analysis of cis-acting elements indicated that there were various cis-acting elements related to stress, development, hormone, and light. The expression profiles showed that most of RcCBFs were mainly expressed in root, and the expression levels of RcCBFs were significantly induced by low-temperature stress, especially RcCBF6. To verify the function of RcCBF6, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The RcCBF6-overexpressing plants exhibited higher tolerance to cold stress as evidenced by a better growth and higher antioxidative enzyme activities than the wild-type plants. Furthermore, the expression levels of some cold-response genes were up-regulated in the transgenic plants, such as KIN1, RD29A, LTP3, and GOLS3. Our study contributes to a better understanding of RcCBF gene family and provides a foundation for the further functional research of RcCBFs.

中文翻译：

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蔷薇CBF基因的鉴定及其RcCBF6在耐冷性中的作用。

C-重复结合因子（CBF）基因家族作为一类AP2家族转录因子，在抵抗低温胁迫和提高植物的耐寒性方面起着重要作用。蔷薇（Rosa chinensis）是重要的园艺和观赏植物，但对R中CBF基因的分子特征了解甚少。中华。在我们的研究中，在R处确定了六个RcCBFs成员。杉全基因组，包括一个构件RcCBF1 1号染色体和其它五个成员RcCBF2 - 6染色体7的分析顺式作用元件表明存在与压力，发育，激素和光有关的各种顺式作用元件。表达谱表明，大多数RcCBFs主要在根中表达，并且低温胁迫特别是RcCBF6明显诱导了RcCBFs的表达水平。为了验证RcCBF6的功能，我们在拟南芥中产生了它的过表达转基因品系。该RcCBF6过表达的植物表现出对冷胁迫的更高的耐受性，这比野生型植物具有更好的生长和更高的抗氧化酶活性所证明。此外，转基因植物中的一些冷响应基因的表达水平也被上调，例如KIN1，RD29A，LTP3和GOLS3。我们的研究有助于更好地了解RcCBF基因家族，并为RcCBFs的进一步功能研究奠定基础。