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Characterization and pathogenicity of Rhizoctonia isolates collected from Brassica oleracea var. acephala in Ordu, Turkey
Phytoparasitica ( IF 1.4 ) Pub Date : 2020-02-24 , DOI: 10.1007/s12600-020-00793-9
Muharrem Türkkan , Melike Çebi Kılıçoğlu , Ismail Erper

A total of 30 Rhizoctonia isolates were collected from plants with Rhizoctonia-like symptoms in kale growing areas in Ordu province during the 2014–2015 growing seasons. All the isolates were identified using nuclear staining tecnique and the nucleotide sequence analysis of the nuclear rDNA internal transcribed spacer (ITS)1–5.8S-ITS2 region. The most prevalent anastomosis group (AG) was AG 2–1 (36.67% of isolates), followed by AG-A (30%), AG 4 HG-I (10%), AG 5 (6.67%), AG-Fb (6.67%), AG-E (6.67%) and AG-K (3.33%). Cultural characteristics showed that randomly selected RsCB-13 (AG 2–1), RsCB-20 (AG 4 HG-I), RsCB-21 (AG 5), RCB-16 (AG-A), RCB-7 (AG-E), RCB-22 (AG-Fb) and RCB-26 (AG-K) isolates representing each AG had greyed-yellow, brown, white-orange and greyed-orange colony colors, and optimum growth temperatures of the isolates ranged from 25.21 to 27.67 °C. Pathogenicity tests on the seedlings of kale cv. Arzuman revealed generally significant virulence differences between AG 2–1 and AG 4 HG-I isolates, and other Rhizoctonia isolates (P < 0.05). In addition, with the exception of several AG 2–1 isolates, the isolates of both groups caused severe root and stem rot and seedling deaths, unlike the relatively low and moderate virulent AG 5, AG-E, AG-Fb and AG-K isolates. However, AG-A had very low virulence and did not affect plant growth parameters such as plant height, shoot and root dry weights and root length while the isolates of all other groups generally reduced these parameters compared to control plants (P < 0.05). To our knowledge, this is the first study reporting AGs of Rhizoctonia isolates causing root and stem rot on kale plants in Turkey and in the world.

中文翻译:

从芸苔中收集的丝核菌分离物的特征和致病性。土耳其奥尔杜的 acephala

在 2014-2015 年生长季节从奥尔杜省羽衣甘蓝种植区的具有丝核菌样症状的植物中收集了总共 30 株丝核菌分离株。使用核染色技术和核 rDNA 内部转录间隔区 (ITS)1-5.8S-ITS2 区域的核苷酸序列分析鉴定所有分离株。最普遍的吻合组 (AG) 是 AG 2–1(分离株的 36.67%),其次是 AG-A(30%)、AG 4 HG-I(10%)、AG 5(6.67%)、AG-Fb (6.67%)、AG-E (6.67%) 和 AG-K (3.33%)。文化特征显示随机选择的 RsCB-13 (AG 2–1)、RsCB-20 (AG 4 HG-I)、RsCB-21 (AG 5)、RCB-16 (AG-A)、RCB-7 (AG- E)、代表每个 AG 的 RCB-22 (AG-Fb) 和 RCB-26 (AG-K) 分离株具有灰黄色、棕色、白橙色和灰橙色菌落颜色,分离株的最佳生长温度范围为25. 21 至 27.67 °C。羽衣甘蓝幼苗的致病性试验。Arzuman 揭示了 AG 2-1 和 AG 4 HG-I 分离株与其他丝核菌分离株之间普遍存在显着的毒力差异(P < 0.05)。此外,除了几个 AG 2-1 分离株外,与相对低毒和中等毒力的 AG 5、AG-E、AG-Fb 和 AG-K 不同,两组的分离株都造成了严重的根茎腐烂和幼苗死亡。隔离。然而,AG-A 的毒力非常低,不影响植物生长参数,如植物高度、地上部和根干重和根长,而所有其他组的分离物与对照植物相比通常降低这些参数(P < 0.05)。据我们所知,这是第一项研究报告了在土耳其和世界上导致羽衣甘蓝植物根和茎腐烂的丝核菌分离株的 AG。羽衣甘蓝幼苗的致病性试验。Arzuman 揭示了 AG 2-1 和 AG 4 HG-I 分离株与其他丝核菌分离株之间普遍存在显着的毒力差异(P < 0.05)。此外,除了几个 AG 2-1 分离株外,与相对低毒和中等毒力的 AG 5、AG-E、AG-Fb 和 AG-K 不同,两组的分离株都造成了严重的根茎腐烂和幼苗死亡。隔离。然而,AG-A 的毒力非常低,不影响植物生长参数,如植物高度、地上部和根干重和根长度,而所有其他组的分离物与对照植物相比通常降低这些参数(P < 0.05)。据我们所知,这是第一项研究报告了在土耳其和世界上导致羽衣甘蓝植物根和茎腐烂的丝核菌分离株的 AG。羽衣甘蓝幼苗的致病性试验。Arzuman 揭示了 AG 2-1 和 AG 4 HG-I 分离株与其他丝核菌分离株之间普遍存在显着的毒力差异(P < 0.05)。此外,除了几个 AG 2-1 分离株外,与相对低毒和中等毒力的 AG 5、AG-E、AG-Fb 和 AG-K 不同,两组的分离株都造成了严重的根茎腐烂和幼苗死亡。隔离。然而,AG-A 的毒力非常低,不影响植物生长参数,如植物高度、地上部和根干重和根长,而所有其他组的分离物与对照植物相比通常降低这些参数(P < 0.05)。据我们所知,这是第一项研究报告了在土耳其和世界上导致羽衣甘蓝植物根和茎腐烂的丝核菌分离株的 AG。
更新日期:2020-02-24
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