The aim of the present study is to develop a surface plasmon resonance sensor for the detection of vitamin B2, vitamin B9, and vitamin B12 in food samples by using the molecular imprinting technique. The vitamin B2, vitamin B9, and vitamin B12 imprinted and the non-imprinted surface plasmon resonance sensor chip surfaces were characterized by using contact angle measurements, atomic force microscopy, ellipsometry, and Fourier transform infrared-attenuated total reflectance. The real-time detection of vitamin B2, vitamin B9, and vitamin B12 was analyzed by using aqueous solutions in the concentration range of 0.01 ng/mL − 10 ng/mL for vitamin B2, 0.1 ng/mL − 8.0 ng/mL for vitamin B9, and 0.01 ng/mL − 1.5 ng/mL for vitamin B12. The limit of detection values was calculated as 1.6×10⁻⁴ ng/mL for vitamin B2, 13.5×10⁻⁴ ng/mL for vitamin B9, and 2.5×10⁻⁴ ng/mL for vitamin B12, respectively. Selectivity experiments were performed by using vitamin B1 and vitamin B6. The reproducibility of surface plasmon resonance sensors was investigated both on the same day and on different days for four times. Validation studies of the prepared surface plasmon resonance (SPR) sensors were performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

本研究的目的是开发一种表面等离子体共振传感器，通过使用分子印迹技术来检测食品中的维生素B2，维生素B9和维生素B12。维生素B2，维生素B9和维生素B12的印迹和非印迹的表面等离振子共振传感器芯片表面通过使用接触角测量，原子力显微镜，椭圆偏振法和傅里叶变换红外衰减的全反射率进行表征。通过使用浓度范围为0.01 ng / mL-10 ng / mL的维生素B2、0.1 ng / mL-8.0 ng / mL的水溶液分析维生素B2，维生素B9和维生素B12的实时检测B9，以及维生素B12的0.01 ng / mL-1.5 ng / mL。检测值的极限计算为1.6×10 ^-4维生素B2为ng / mL，维生素B9为13.5×10 ^-4 ng / mL，维生素B12为2.5×10 ^-4 ng / mL。通过使用维生素B1和维生素B6进行选择性实验。在同一天和不同天均对表面等离子体共振传感器的可重复性进行了四次研究。通过液相色谱-串联质谱（LC-MS / MS）对制备的表面等离子体共振（SPR）传感器进行了验证研究。