The effect of using encapsulated shoot tips (ST) and nodal segment (NS) explants, in combination with mineral oil (MO) overlay, for in vitro regeneration and efficient midterm conservation of Bacopa monnieri (IC468878), a medicinal herb with potent nervine properties is described. On a standard multiplication medium [Murashige and Skoog (MS) containing 0.2 mg l⁻¹ benzyladenine (BA)] after 8 wk of culture, the number of shoots regenerated from ST (17.50 ± 0.50) to NS (19.10 ± 1.20) were not significantly different; however, the shoot length regenerated from NS (7.30 ± 0.70 cm) was significantly higher than ST (2.50 ± 0.30 cm). Encapsulation of ST explants did not have any significant effect on the number of shoots (18.10 ± 1.00) and shoot length (3.50 ± 0.30 cm), while there was significant decrease in shoot number (10.30 ± 1.0) and shoot length (1.50 ± 0.40 cm) in NS explants. In general, use of MO overlay resulted in highly significant reduction of growth and increase in survival of cultures. The combined strategy of encapsulation and MO overlay led to growth reduction in terms of number of shoots (2.50 ± 0.30 in ST 2.30 ± 0.20 in NS) as well as shoot length (0.50 ± 0.02 cm in both explants). After a 12-mo in vitro conservation, encapsulated NS explants in combination with MO overlay gave the highest survival rate (91.6 ± 5.7%) and number of shoots (21.4 ± 1.6). This is the first report on success of combining two slow-growth strategies of reduced oxygen environment and synseed technology for midterm (12 mo) in vitro conservation of germplasm.

使用包封的茎尖（ST）和节点段（NS）的外植体，在组合与矿物油（MO）覆盖，所获得效果的体外再生和高效的中期保护假马齿苋（IC468878），药草，具有强有镇定作用的性质描述。在标准繁殖培养基上[含有0.2 mg l ^-1的Murashige和Skoog（MS）培养8周后，从ST（17.50±0.50）再生为NS（19.10±1.20）的芽数没有显着差异。然而，从NS（7.30±0.70 cm）再生的枝条长度显着高于ST（2.50±0.30 cm）。ST外植体的包封对枝条数（18.10±1.00）和枝条长度（3.50±0.30 cm）没有明显影响，而枝条数（10.30±1.0）和枝条长度（1.50±0.40）却没有显着降低厘米）在NS外植体中。通常，MO覆盖物的使用导致生长的显着降低和培养物存活率的显着降低。包囊和MO覆盖相结合的策略导致枝条数量减少（ST中为2.50±0.30，ST中为2.30±0.20，在NS中）以及枝条长度（两种外植体均为0.50±0.02 cm）。在体外保存中，囊封的NS外植体与MO覆盖层结合可提供最高的成活率（91.6±5.7％）和枝条数（21.4±1.6）。这是首次成功地将两种降低氧气环境的慢速生长策略与合成技术相结合，成功地中期（12 mo）用于种质的体外保存。