The synergistic effect of plant growth regulators on axillary bud proliferation for mass clonal multiplication of Moringa oleifera Lam. (vern. drumstick) has been assessed for the first time. Treatment of decoated seeds with 1% (w/v) Bavistin for 60 min, 0.33% (w/v) streptocycline for 30 min, and 0.1% (w/v) HgCl₂ for 3.5 min resulted in complete removal of the surface contaminants. Maximum seed germination (89.13%) was obtained on quarter-strength Murashige & Skoog (MS) medium. Culture of nodal segments on MS + 6-benzyladenine (BA) at 3 mg L⁻¹ resulted in multiple shoot proliferation with ~ 18 shoots per explant. All combinations of indole-3-acetic acid (IAA) + kinetin (Kn) resulted in elongated shoots, while only lower concentrations of BA (0.5 mg L⁻¹), along with IAA (0.5 to 2 mg L⁻¹), or Kn (0.5 to 5 mg L⁻¹), showed significant synergy in the shoot morphogenesis. In addition, the maximum (100%) rooting efficiency was attained on half-strength MS medium supplemented with different concentrations of IAA and indole-3-butyric acid (IBA). The rooted plants were successfully established in the greenhouse for acclimatization. Clonality of the raised plants was assessed using 15 random primers of Operon® technologies (OPT and OPF series), and eight primers resulted in significant amplification with distinct, identical, and reproducible bands that confirmed clonality of the micropropagated plants. The present study provides a comprehensive analysis of the synergistic effect of plant growth regulators (PGRs) on in vitro shoot regeneration and proliferation for clonal mass multiplication disease-free plantlets, which can be utilized to maximize the yield of healthy and genetically identical plants of drumstick tree, which is considered to be a miracle multipurpose tree.

辣木大规模克隆繁殖中植物生长调节剂对腋芽增殖的协同作用。（vern。鼓槌）已进行首次评估。去除涂层的种子的处理，用1％（w / v的）Bavistin 60分钟，0.33％（w / v的）streptocycline 30分钟，和0.1％（w / v的）的HgCl ₂，3.5分钟导致完全除去表面污染物。在四分之一强度的Murashige＆Skoog（MS）培养基上可获得最大的种子发芽率（89.13％）。在MS + 6-苄基腺嘌呤（BA）上以3 mg L ^-1培养节点节段导致多芽繁殖，每个外植体约18个芽。吲哚-3-乙酸（IAA）+激动素（Kn）的所有组合均导致芽伸长，而只有较低浓度的BA（0.5 mg L ^-1）和IAA（0.5至2 mg L ^-1），或Kn（0.5至5 mg L ^-1），在芽的形态发生中表现出明显的协同作用。此外，在半强度MS培养基中添加不同浓度的IAA和吲哚-3-丁酸（IBA）可获得最大（100％）生根效率。生根的植物已成功地建立在温室中以适应环境。使用15种Operon®技术（OPT和OPF系列）随机引物评估了育苗植物的克隆性，八种引物以明显，相同和可再现的条带显着扩增，证实了微繁殖植物的克隆性。本研究提供了植物生长调节剂（PGRs）对体外协同作用的综合分析 克隆繁殖无病小植株的芽再生和增殖，可用于最大化鼓槌树的健康和遗传上相同的植物的产量，鼓槌树被认为是奇迹多用途树。