There is little information on the in vitro tissue culture systems in Lamprocapnos spectabilis (bleeding heart). The aim of this study was to analyze the influence of plant growth regulators (PGRs) on the development, quality, and physiological state of in vitro-grown bleeding heart “Gold Heart” and “White Gold”. Single-node explants were inoculated on the modified MS medium (Murashige and Skoog in Physiol Plant 15:473–497, 1962), fortified with different auxins, which included indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and picloram (PIC), along with cytokinins, which included 6-benzyladenine (BA), kinetin (KIN), and thidiazuron (TDZ) at various concentrations. The morphogenetic response of the explants was cultivar-specific. KIN was preferable for the proliferation and development of shoots in “Gold Heart.” However, none of the auxins or cytokinins improved the development of “White Gold” explants, compared with the PGR-free control medium. NAA was the most effective for stimulating rhizogenesis in both cultivars, although IAA resulted in the regeneration of the longest roots. TDZ, NAA, and PIC suppressed the development of shoots in both cultivars tested and stimulated abundant callus formation. Indirect regeneration of somatic embryos occurred on the NAA- and PIC-fortified media. In particular, the latter media stimulated regeneration of the highest number of somatic embryos per nodal segment. Composition of the culture medium also affected the levels of primary and secondary metabolites in shoots and callus of L. spectabilis. IAA (at 1.0 mg L⁻¹) stimulated the synthesis of chlorophyll a and carotenoids in the “Gold Heart,” while BA and KIN (at 0.5 mg L⁻¹) had a negative impact on the concentration of chlorophyll b in the shoots of this cultivar. None of the PGRs increased the level of the pigments in the shoots of bleeding heart “White Gold.” The concentration of chlorophylls and carotenoids in the callus of both cultivars tested was significantly lower compared with the shoots; however, callus was abundant in flavanols.

关于小球藻（心脏出血）的体外组织培养系统的信息很少。这项研究的目的是分析植物生长调节剂（PGR）对体外培养的发育，质量和生理状态的影响。成长的流血心脏“金心”和“白金”。将单节点外植体接种在改良的MS培养基上（Murashige和Skoog在Physiol Plant 15：473-497，1962年）中接种，其中加入了不同的生长素，其中包括吲哚-3-乙酸（IAA），1-萘乙酸（NAA）。 ），吡咯烷酮（PIC）以及细胞分裂素，其中包括各种浓度的6-苄腺嘌呤（BA），激动素（KIN）和噻唑酮（TDZ）。外植体的形态发生反应是特定品种的。对于“金心”中芽的增殖和发育，KIN是优选的。但是，与不含PGR的对照培养基相比，生长素或细胞分裂素均未改善“白金”外植体的发育。尽管IAA导致最长根的再生，但NAA在刺激两个品种的根际发生方面最有效。TDZ，NAA，PIC抑制了两个试验品种的芽发育，并刺激了大量的愈伤组织形成。体细胞胚的间接再生发生在NAA和PIC强化培养基上。特别地，后一种培养基刺激每个结节节段最高数量的体细胞胚的再生。培养基的组成也影响了芽和愈伤组织中初级和次级代谢产物的水平。L. spectabilis。IAA（1.0 mg L ^-1）刺激了“金心”中叶绿素a和类胡萝卜素的合成，而BA和KIN（0.5 mg L ^-1）对绿豆芽中叶绿素b的浓度有负面影响。这个品种。没有一种植物遗传资源会增加心脏“白金”出血枝条中色素的含量。与芽相比，所测试的两个品种的愈伤组织中的叶绿素和类胡萝卜素的浓度均显着降低。但是，愈伤组织中含有丰富的黄烷醇。