Plectranthus amboinicus (Loureiro) Sprengel is a therapeutically valued herb highly sought by various industries. This has led to its uncontrolled harvest from the wild leading to the risk of extinction. As an alternative, commercial propagation of P. amboinicus is desirable to avoid further depletion of wild populations. This paper describes the establishment of an efficient protocol for the multiplication, rooting and acclimatization of P. amboinicus. The greatest frequency of shoot multiplication of P. amboinicus was on semi-solid Murashige and Skoog (MS) medium supplemented with 0.4 mg L⁻¹ 6-benzylaminopurine (BAP). Media supplemented with higher concentrations of BAP or used in combination with gibberellic acid (GA₃) produced abnormal plantlets. Optimal rooting was observed on plantlets cultured in half strength MS medium. Acclimatization was achieved by transferring the rooted plants onto sterile peat-moss moistened with MS medium and exposed to the glasshouse environment gradually over a period of 2 months. P. amboinicus responded readily to tissue culture, but careful attention was required to media formulation to avoid abnormal plantlet development. The composition of essential oils extracted from field grown plants and in vitro microshoots were comparable.

Plectranthus amboinicus（Loureiro）Sprengel是一种具有治疗价值的草药，受到各行各业的追捧。这导致其从野外的不受控制的收成导致灭绝的风险。作为替代方案，理想的是将P.amboinicus进行商业繁殖，以避免野生种群进一步枯竭。本文介绍了一种有效的协议，用于竹节虫的繁殖，生根和驯化。杆状假单胞菌的芽繁殖的最大频率是在补充有0.4 mg L ^-1 6-苄基氨基嘌呤（BAP）的半固态Murashige和Skoog（MS）培养基上。补充了更高浓度的BAP或与赤霉素（GA₃）产生异常的幼苗。在半强度MS培养基中培养的小植株上观察到最佳的生根。通过将生根的植物转移到用MS培养基浸湿的无菌泥炭藓中，并在2个月的时间内逐渐暴露于温室环境中，从而实现了驯化。南美白对虾对组织培养很容易做出反应，但需要特别注意培养基的配制，以免植物幼苗发育异常。从田间生长的植物中提取的精油的成分和体外微芽的含量可比。