Sugarcane is a very important crop in tropical areas around the world. Optimizing the light intensity and culture vessel for sugarcane in vitro propagation can improve protocols for large-scale in vitro mass clonal propagation. The objective of this study was to evaluate biometric and anatomical characteristics of in vitro propagated sugarcane plantlets using different light intensities and culture vessel types. The study also aimed to stimulate desired photoautotrophic and/or photomixotrophic traits and to study correlations between survival and ex vitro vigor with in vitro data. Fluorescent lights at 70 μmol m⁻² s⁻¹ and light-emitting diodes (LEDs) at 120 and 200 μmol m⁻² s⁻¹ were used combined with different culture vessel types, including microboxes without a filter, microboxes with a filter, and baby food jars. Propagation and rooting growth were observed in all in vitro culture conditions. However, an increase in light intensity and change of culture vessel type provided improved results. When sugarcane plantlets were cultivated within microboxes with a filter under 120 and 200 μmol m⁻² s⁻¹, they showed improved growth, increased chlorophyll content, the lowest leaf yellowing percentage, an increase in bulliform cells and, consequently, increased leaf thickness under in vitro culture. After 60 d in greenhouse conditions, in vitro-derived plantlets under LEDs and microbox culture vessels showed the highest biometric values and high ex vitro survival.

甘蔗是世界热带地区非常重要的作物。优化甘蔗体外繁殖的光强度和培养容器可以改善大规模体外大规模克隆繁殖的方案。这项研究的目的是使用不同的光强度和培养容器类型来评估体外繁殖的甘蔗苗的生物特征和解剖特征。该研究还旨在刺激所需的光合自养和/或光合养分性状，并利用体外数据研究存活率和离体活力之间的相关性。70μmolm ^-2  s ^-1的荧光灯将120和200μmolm ^-2  s ^-1的发光二极管（LED）与不同的培养容器类型结合使用，包括不带过滤器的微箱，带过滤器的微箱和婴儿食品罐。在所有体外培养条件下均观察到繁殖和生根生长。然而，光强度的增加和培养容器类型的改变提供了改善的结果。当在120和200μmolm ^-2  s ^-1以下的过滤器的微箱中种植甘蔗苗时，它们显示出生长的改善，叶绿素含量的增加，叶黄变的百分比最低，花状细胞的增加以及因此在以下条件下叶厚度的增加。体外文化。在温室条件下60 d后，在LED和微箱培养皿下的体外衍生苗显示出最高的生物测定值和较高的离体存活率。