Transformed hairy root cultures have become an alternative for the biosynthesis of plant secondary metabolites with biological activities. In this present work, the effects of liquid Murashige and Skoog (MS) and Gamborg B5 (B5) medium on kinetic behavior, biomass and phenolic metabolite production were analyzed in Turbinicarpus lophophoroides (Werderm.) Buxb. & Backeb. hairy root cultures. Liquid MS medium showed the highest biomass production (13.67 g L⁻¹ dry weight) after 77 d of culture. For B5 medium, highest biomass was achieved sooner, at day 56, but with lower total biomass (8.10 g L⁻¹ dry weight). After isolation, structural elucidation of the major compound present in T. lophophoroides hairy roots was determined by nuclear magnetic resonance and mass spectral analysis. As a result, a ferulic acid derivative (feruloyl-glucoside) was isolated from T. lophophoroides hairy roots and reported for the first time. Quantitative analysis indicated that feruloyl-glucoside was the major phenolic metabolite at 56 d of growth in MS medium (2.7267 ± 0.041 mg g⁻¹ dry weight L⁻¹) and at 7 and 35 d in B5 medium (2.6328 ± 0.108 and 2.4372 ± 0.026 mg g⁻¹ dry weight L⁻¹, respectively). The feruloyl-glucoside was not detected in untransformed roots (control). The present results suggested the potential of T. lophophoroides hairy roots culture for the production of this phenolic glycoside.

转化的毛状根培养物已成为具有生物活性的植物次生代谢产物生物合成的替代方法。在本工作中，分析了Turbinicarpus lophophoroidoides（Werderm。）Buxb中液体Murashige和Skoog（MS）和Gamborg B5（B5）介质对动力学行为，生物量和酚类代谢产物产生的影响。＆Backeb。毛根文化。液体MS培养基在培养77天后显示出最高的生物量产量（13.67 g L ^-1干重）。对于B5培养基，在第56天更快地获得了最高的生物量，但总生物量却较低（8.10 g L ^-1干重）。分离后，对T. lophophoroides中存在的主要化合物进行结构解析通过核磁共振和质谱分析确定毛状根。结果，从T. lophophoroides毛状根中分离出阿魏酸衍生物（阿魏酸-葡萄糖苷）并首次报道。定量分析表明，在MS培养基（2.7267±0.041 mg g ^-1干重L ^-1）生长56 d和B5培养基（2.6328±0.108和2.4372±分别为0.026 mg g ^-1干重L ^-1）。在未转化的根中未检测到阿魏酰葡萄糖苷（对照）。目前的结果表明，T。lophophoroides的潜力 毛状根培养物中可产生这种酚糖苷。