One of the strategies for genetic improvement of Oryza sativa L. (rice) is the use of radiation-induced mutagenesis to search for materials suitable for production in marginal conditions, such as drought and salinity. In this present study, indica rice ‘CR-5272’ embryogenic calluses and seeds were exposed to gamma radiation. Survival and growth of plants derived from embryogenic calluses and seeds were evaluated in vitro in the presence of selection factors for salinity and drought (NaCl and sorbitol). Plants regenerated from irradiated calluses and seeds were screened for tolerance to these conditions and promising lines were identified. Culture of plants regenerated from irradiated calluses (M1) allowed the selection of 60, 58 and 15 promising lines with tolerance to salinity, drought, and both factors, respectively. Six promising salt-tolerant lines and four drought-tolerant lines were selected from plants grown from irradiated seeds (M2). A hormetic response to irradiation was observed in seeds; seeds irradiated at 50 Gy showed increased callus induction and callus weight. This study allowed advancements in the improvement of rice ‘CR-5272’ through the production of promising lines that are being evaluated in field studies.

水稻遗传改良的策略之一是利用辐射诱变来寻找适合在干旱和盐碱等边际条件下生产的材料。在本研究中，in稻'CR-5272'胚性愈伤组织和种子暴露于伽玛射线。体外评估了源自胚性愈伤组织和种子的植物的存活和生长存在盐分和干旱的选择因子（NaCl和山梨糖醇）。筛选从辐照的愈伤组织和种子再生的植物对这些条件的耐受性，并鉴定出有希望的品系。从辐照的愈伤组织（M1）再生的植物培养物可以分别选择60、58和15个有前途的耐盐，干旱和这两个因素的品系。从辐照种子（M2）生长的植物中选择了六个有希望的耐盐品系和四个耐旱品系。在种子中观察到对辐射的兴奋反应；50 Gy辐照的种子显示出愈伤组织诱导和愈伤组织重量增加。这项研究通过在田间研究中评估有前途的品系，使水稻'CR-5272'的改良取得了进展。