The aim of this work was to demonstrate the production of polyol from microbial oil using wood-based hemicellulose stream through a greener enzymatic process. Yeast biomass concentration of 16.54 ± 0.65 g/L and lipid concentration of 6.97 ± 0.58 g/L were obtained by batch fermentation of hemicellulose prehydrolysate of poplar wood using an oleaginous yeast Cryptococcus curvatus. The produced microbial oil was successfully converted to epoxidized oil catalyzed by lipase with 84.55 ± 1.80% conversion. The epoxidation followed by ring opening reaction to produce polyol with hydroxyl value of 299.53 ± 1.24 mg KOH/g. This showed the possible use of the hemicellulose stream of lignocellulosic biomass to microbial oil and its subsequent conversion to polyol, a precursor to polyurethane and a number of value-added products. This bio-based polyol could be used to substitute the conventional polyols.

这项工作的目的是证明通过使用木基半纤维素流通过绿色的酶促工艺从微生物油中生产多元醇。通过使用油性酵母曲霉（Cryptococcus curvatus）分批发酵杨木的半纤维素预水解物，可获得酵母生物量浓度为16.54±0.65 g / L和脂质浓度为6.97±0.58 g / L。产生的微生物油被脂肪酶催化成功转化为环氧化油，转化率为84.55±1.80％。进行环氧化后进行开环反应，制得羟值为299.53±1.24 mg KOH / g的多元醇。这表明可能将木质纤维素生物质的半纤维素物流用于微生物油，并随后将其转化为多元醇，聚氨酯的前体和许多增值产品。这种生物基多元醇可以用来代替常规多元醇。