Stress is an emerging and inevitable entity. Stress causes anxiety, depression, imbalance of homeostasis, hormone imbalance, and sleep disturbances that are closely related to the onset of metabolic syndrome. The objective of this study was to investigate effects of Ocimum sanctum extract (TZ-OSE) on anti-anxiety, anti-stress, and control of physiological changes due to sleep disturbance in a rat model with restraint stress and sleep disorder induced. After inducing the rat model with restraint stress, three behavioral tests were performed and blood cortisol concentration was determined. In Elevated plus maze test, the test substance TZ-OSE at dose of 200 mg/kg showed the best effect in increasing time that stayed on the open arms. In tail suspension test (TST) and forced swimming test (FST), TZ-OSE significantly reduced immobility duration time (IMT). TZ-OSE also decreased plasma cortisol concentration. In the model after inducing sleep disturbance, ELISA, Western blotting, and Immuno-histochemistry (IHC) were conducted. TZ-OSE treatment decreased plasma cortisol, norepinephrine, glucose, and acylated ghrelin concentrations in the rat model with sleep disorders induced. Decreases of melatonin and leptin levels in the plasma of the rat model with sleep disorders induced were increased by TZ-OSE treatment. Finally, TZ-OSE treatment decreased metabolism-related markers (p-AMPK, Glut-4, p-ACC) in stomach and muscle tissues and decreased the expression of cortisol in adrenal gland tissues. Taken together, these results suggest that TZ-OSE can decrease anxiety, depression, and stress in vivo, regulate sleep-related factors, and control energy metabolism index factors.

压力是一个新兴且不可避免的实体。压力会导致焦虑，沮丧，体内平衡失衡，激素失衡和睡眠障碍，这些与代谢综合征的发作密切相关。这项研究的目的是调查圣坛的影响提取物（TZ-OSE）对具有约束性压力和睡眠障碍的大鼠模型具有抗焦虑，抗压力和控制由于睡眠障碍引起的生理变化的作用。在用约束压力诱导大鼠模型后，进行了三个行为测试并确定了血液中的皮质醇浓度。在高架迷宫测试中，剂量为200 mg / kg的被测物质TZ-OSE在延长手臂停留时间方面显示出最佳效果。在尾部悬挂测试（TST）和强制游泳测试（FST）中，TZ-OSE显着减少了固定持续时间（IMT）。TZ-OSE还降低血浆皮质醇浓度。在诱发睡眠障碍的模型中，进行了ELISA，Western印迹和免疫组织化学（IHC）。TZ-OSE治疗可降低血浆皮质醇，去甲肾上腺素，葡萄糖，诱发睡眠障碍的大鼠模型中的生长激素和酰化生长素释放肽浓度。TZ-OSE治疗可增加诱发睡眠障碍的大鼠模型血浆中褪黑激素和瘦素水平的降低。最后，TZ-OSE治疗降低了胃和肌肉组织中与代谢相关的标志物（p-AMPK，Glut-4，p-ACC），并降低了肾上腺组织中皮质醇的表达。综上所述，这些结果表明TZ-OSE可以减轻焦虑，抑郁和压力 p-ACC）并降低肾上腺组织中皮质醇的表达。综上所述，这些结果表明TZ-OSE可以减轻焦虑，抑郁和压力 p-ACC）并降低肾上腺组织中皮质醇的表达。综上所述，这些结果表明TZ-OSE可以减轻焦虑，抑郁和压力在体内，调节睡眠相关因素，并控制能量代谢指数因素。