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Does temperature affect the occurrence of penaeid spermatophore melanization related to successive manual extrusions? A case study with the closed thelycum Farfantepenaeus paulensis
Aquaculture International ( IF 2.9 ) Pub Date : 2020-04-18 , DOI: 10.1007/s10499-020-00549-6
Cintia Labussière Nakayama , Diogo Lopes , André Braga , Wilson Wasielesky , Ronaldo O. Cavalli

Spermatophore successive manual extrusions are often employed in hatcheries, but it causes melanin deposition, decreasing sperm quality via cell degeneration. Melanization (brown or black spots) is an indicator of the moment to discard males. The evaluation of more efficient strategies is required to avoid it, increasing males’ lifespan. The effects of two temperatures (26 and 30 °C) on closed thelycum Farfantepenaeus paulensis spermatophore melanization and sperm quality after successive manual extrusions were evaluated. Wild-caught males were acclimated to laboratory conditions for 1 week and fed commercial broodstock feed and fresh-frozen food items ad libitum four times a day. Later, a 35-day trial was conducted with 24 males (26.05 ± 2.4 g) randomly distributed into eight 200-L tanks (4.3 shrimps m−2). Temperature was gradually increased from 23 to 26 °C or 30 °C. Only males showing a formed spermatophore and in the intermolt period were submitted to four consecutive manual extrusions every 11 days. Melanization was visually examined in extruded spermatophores, which were weighed. Spermatozoa were released in saline solution and observed in a hemacytometer under a light microscope. Morphological abnormalities (main body or spike malformations) were identified. Total and abnormal spermatozoa were counted in five fields of the hemacytometer to calculate sperm count and abnormal sperm rate. No significant differences were found on sperm quality among extrusions, but from the second extrusion onwards, an increase of melanization was evident. On the other hand, temperature did not affect the occurrence of spermatophore melanization related to successive extrusions.



中文翻译:

温度会影响与连续手动挤压有关的对虾精子黑色素化的发生吗?封闭的茶花草Farfantepenaeus paulensis的案例研究

孵化场经常使用精子连续人工挤压,但会导致黑色素沉积,通过细胞变性降低精子质量。变黑(褐色或黑色斑点)是丢弃雄性动物的时机指标。为了避免这种情况,需要评估更有效的策略,以延长男性的寿命。在连续手动挤压后,评估了两个温度(26和30°C)对封闭的食堂Farfantepenaeus paulensis精子黑色素化和精子质量的影响。野外捕获的雄性在实验室条件下适应了1周,每天随意四次喂食商业亲鱼饲料和新鲜冷冻食品。之后,进行了为期35天的试验,将24只雄性(26.05±2.4 g)随机分配到八个200 L的水箱中(4.3虾米−2)。温度逐渐从23°C升高到26°C或30°C。每11天,只有表现出精子形成并处于蜕膜期的雄性接受四次连续手动挤压。肉眼检查挤压的精原细胞,然后称重。精子在盐溶液中释放,并在血细胞计数器中在光学显微镜下观察。确定了形态异常(主体或峰形畸形)。在血球计数器的五个区域中对精子的总数和异常计数,以计算精子数量和异常精子率。挤压之间的精子质量没有显着差异,但是从第二次挤压开始,黑色素的增加是明显的。另一方面,

更新日期:2020-04-18
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