Hydrogen sulfide (H₂S) is a highly poisonous gas with an unpleasant smell of rotten eggs. Previous studies of H₂S have primarily focused on its effects on mammalian nervous and respiratory systems. In this study, silkworm developmental parameters and changes in metabolites in response to H₂S exposure were investigated using a hemolymph metabolomic approach, based on liquid chromatography–mass spectrometry (LC-MS). The developmental parameters, body weight, cocoon weight, cocoon shell weight, and cocoon shell ratio, were noticeably increased following H₂S exposure, with the greatest effects observed at 7.5-μM H₂S. Metabolites upregulated under H₂S exposure (7.5 μM) were related to inflammation, and included (6Z, 9Z, 12Z)-octadecatrienoic acid, choline phosphate, and malic acid, while hexadecanoic acid was downregulated. Identified metabolites were involved in biological processes, including pyrimidine, purine, and fatty acid metabolism, which are likely to affect silk gland function. These results demonstrate that H₂S is beneficial to silkworm development and alters metabolic pathways related to spinning function and inflammation. The present study provides new information regarding the potential functions of H₂S in insects and metabolic pathways related to this phenomenon.

中文翻译：

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基于液相色谱-质谱的代谢组学评估硫化氢对家蚕（鳞翅目：家蝇科）发育的代谢作用。

硫化氢（H ₂ S）是一种剧毒气体，带有难闻的腐烂鸡蛋味。H ₂ S的先前研究主要集中于其对哺乳动物神经和呼吸系统的影响。在这项研究中，基于液相色谱-质谱法（LC-MS），使用血淋巴代谢组学方法研究了家蚕的发育参数和响应H ₂ S暴露的代谢物变化。暴露于H ₂ S后，发育参数，体重，茧重，茧壳重量和茧壳比率显着增加，在7.5-μMH ₂ S处观察到最大的影响。在H ₂下代谢产物上调S暴露（7.5μM）与炎症有关，包括（6 Z，9 Z，12 Z）-十八碳三烯酸，磷酸胆碱和苹果酸，而十六烷酸被下调。鉴定出的代谢物参与了生物过程，包括嘧啶，嘌呤和脂肪酸代谢，它们可能会影响丝腺功能。这些结果表明，H ₂ S有利于家蚕发育并改变与纺丝功能和炎症相关的代谢途径。本研究提供有关H ₂ S在昆虫中的潜在功能以及与此现象有关的代谢途径的新信息。