Goal: Artificially engineering the tumor microenvironment in vitro as a vital tool for understanding the mechanism of tumor progression. In this study, we developed three-dimensional cell scaffold systems with different topographical features and mechanical properties but similar surface chemistry. The cell behavior was modulated by the topography and mechanical properties of the scaffold. Methods: Adenocarcinoma (MCF7), triple-negative (MDA-MB-231) and premalignant (MCF10AneoT) breast cancer cells were seeded on the scaffold systems. The cell viability, cell-cell interaction and cell-matrix interactions were analyzed. The preferential growth and alignment of specific population of cells were demonstrated. Results: Among the different scaffolds, triple-negative breast cancer cells preferred honeycomb scaffolds while adenocarcinoma cells favored mesh scaffolds and premalignant cells preferred the aligned scaffolds. Conclusions: The 3D model system developed here can be used to support growth of only specific cell populations or for the growth of tumors. This model can be used for understanding the topographical and mechanical features affecting tumorigenesis, cancer cell growth and migration behavior of malignant and metastatic cancer cells.

中文翻译：

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使用刚度和形态学相关的细胞粘附来调节乳腺癌细胞命运的工程化三维支架

目标：在体外人工工程化肿瘤微环境，作为了解肿瘤进展机制的重要工具。在这项研究中，我们开发了具有不同地形特征和机械性能但表面化学相似的三维细胞支架系统。细胞行为受支架的形貌和机械性能调节。方法：将腺癌 (MCF7)、三阴性 (MDA-MB-231) 和癌前 (MCF10AneoT) 乳腺癌细胞接种到支架系统上。分析细胞活力、细胞-细胞相互作用和细胞-基质相互作用。证明了特定细胞群的优先生长和排列。结果：在不同的支架中，三阴性乳腺癌细胞更喜欢蜂窝支架，而腺癌细胞更喜欢网状支架，癌前细胞更喜欢对齐的支架。结论：这里开发的 3D 模型系统可用于支持特定细胞群的生长或肿瘤的生长。该模型可用于了解影响恶性和转移性癌细胞的肿瘤发生、癌细胞生长和迁移行为的地形和机械特征。