Gynaephora qinghaiensis is a pest on the Qinghai-Tibet Plateau (QTP) that has led to substantial destruction of grassland vegetation. Its pupae are also natural hosts of parasitic wasp. Sexual dimorphism in immune responses is prevalent in vertebrates and invertebrates. However, sexual dimorphism in immune responses of insects, particularly G. qinghaiensis, is poorly understood at the transcriptional level. Here, we performed transcriptome sequencing in male and female pupae of G. qinghaiensis. A total of 118,357,040 clean reads were obtained and assembled into 114,944 unigenes. To explore the difference of immune responses in pupae of both sexes (male and female) of G. qinghaiensis. based on transcriptional level, we characterized the expression profiles of candidate transcripts in the two sexes of G. qinghaiensis. A total of 3,469 unigenes (1,888 up-regulated and 1,581 down-regulated genes) were differentially expressed in pupae of both sexes. Among the differentially expressed genes (DEGs), 263 unigenes related to immune responses were found in the two sexes in G. qinghaiensis; of these, 202 were up-regulated and 61 were down-regulated in the female pupae compared to male pupae. This indicates there were some differences in the expression of immune-related genes between male and female G. qinghaiensis pupae. Therefore, we speculated that the differences in immune responses also likely exsited in pupae of both sexes. Our report provides a valuable genomic resource for further studies of Gynaephora and improves our understanding of the molecular mechanisms underlying immunological differences between male and female insects.

青海龙脑是青藏高原上的一种害虫，已导致草原植被遭到严重破坏。它的p也是寄生黄蜂的天然宿主。免疫反应中的性二态性在脊椎动物和无脊椎动物中很普遍。但是，昆虫的免疫反应中的性二态性，尤其是青海棉在转录水平上了解得很少。在这里，我们对青海G的雄性和雌性up进行了转录组测序。总共获得了118,357,040个干净读物，并将其组装成114,944个单基因。探讨青海。雌雄两性p免疫应答的差异。根据转录水平，我们表征了青海棉the两性中候选转录本的表达谱。共有3469个单基因（1888个上调基因和1581个下调基因）在两性p中差异表达。在差异表达基因（DEGs）中，青海按性别划分有263个与免疫反应有关的单基因。其中，与雄p相比，雌p中有202份上调，有61份下调。这表明青海男性和女性之间免疫相关基因的表达存在一些差异。蛹。因此，我们推测免疫反应的差异也可能在两性p中都存在。我们的报告提供了宝贵的基因组资源，可用于进一步研究绞股蓝，并增进我们对雄性和雌性昆虫免疫学差异的分子机制的了解。