The purpose of this study was to select the most effective Metarhizium anisopliae isolates for the simultaneous control of Aedes albopictus and Culex pipiens mosquito adults. The selection was performed using 65 isolates of M. anisopliae, an entomopathogenic fungus isolated from Korean soils. The selection process was carried out through evaluations of conidial thermotolerance, UV-B tolerance, pathogenicity and virulence against adults of two mosquito species and conidial production on grain medium. The thermotolerance of 65 M. anisopliae isolates ranged from 0% to 99%, and their UV-B tolerance ranged from 7.1% to 94.8%. Among them, 30 isolates with high thermotolerance were selected for evaluation of their pathogenicity and virulence against adults of the two mosquito species. Metarhizium anisopliae isolates were more susceptible to Cx. pipiens adults than Ae. albopictus adults because 11 isolates were pathogenic to Ae. albopictus and 21 isolates were pathogenic to Cx. pipiens. Virulence and conidial production were evaluated using 6 isolates with similar pathogenicity against the two species of mosquito. As a result, the accumulated mortality was 74–100% for Ae. albopictus and 78–100% for Cx. pipiens. LT₅₀ values ranged from 2.3 to 5.2 days for Ae. albopictus and from 3.2 − 4.7 days for Cx. pipiens. Comparison of conidial production using three kinds of grain media showed various results according to isolate and medium. Based on the above results, M. anisopliae CN6S1W1 was finally selected as the most effective fungal isolate for the simultaneous control of Ae. albopictus and Cx. pipiens mosquito adults.

这项研究的目的是选择对白纹伊蚊和淡色库蚊成虫同时进行控制的最有效的金属灭蚊菌。使用来自韩国土壤中的昆虫病原真菌M. anisopliae的65个分离株进行选择。通过评估分生孢子耐热性，对紫外线-B的耐受性，对两种蚊虫成虫的致病性和毒力以及在谷物培养基上的分生孢子产生来进行选择过程。65 M. anisopliae的耐热性分离物的范围从0％到99％，其UV-B耐受性范围从7.1％到94.8％。其中，选择了30株具有高耐热性的菌株，以评估它们对这两种蚊子成虫的致病性和毒力。拟南芥分离株更易感染Cx。pipiens的成年人比Ae大。白化病成虫，因为有11种分离物对Ae具有致病性。白化病和21种分离株对Cx有致病性。pipiens。使用6种对两种蚊子具有相似致病性的分离株评估毒力和分生孢子。结果，Ae的累积死亡率为74–100％。白纹伊蚊和78-100％CX。pipiens。Ae的LT ₅₀值范围从2.3到5.2天。白化病，Cx从3.2到4.7天。pipiens。使用三种谷物培养基进行分生孢子生产的比较显示，根据分离物和培养基，结果不同。基于以上结果，最终选择了M. anisopliae CN6S1W1作为同时控制Ae的最有效真菌分离株。albopictus和Cx。pipiens蚊子成虫。