Demand for fungal xylanases in industrial biotechnological processes shows a clear increase worldwide, so there is an interest in adjusting the conditions of microbial xylanases production. In this study, the ability of the fungus Fusarium solani to produce extracellular xylanases with low cellulolytic activity was optimized by Box Wilson design. The best culture conditions were determined to obtain a crude enzyme preparation with significant xylanolytic activity and little cellulolytic activity. In most treatments, the xylanolytic activity was higher than the cellulolytic activity. A negative effect on the production of endoxylanases, β-xylosidases and endocellulases was observed with the increasing of xylan concentration. Increasing the incubation time adversely affected the production of endocellulases and β-xylosidases. According to the mathematical model and experimental tests, it is possible to produce endoxylanases with minimal endocellulase activity increasing incubation time and the concentration of ammonium sulfate. The optimal culture conditions to produce a greater amount of endoxylanases (10.65 U/mg) and low endocellulases from F. solani were: 2.5% (w/v) xylan, 5.0, 2.0 and 0.4 g/l, of yeast extract, ammonium sulfate and urea, respectively, with 120 h of incubation.

工业生物技术过程中对真菌木聚糖酶的需求在全球范围内显着增加，因此人们对调整微生物木聚糖酶的生产条件很感兴趣。在这项研究中，真菌Fusarium solani的能力Box Wilson设计优化了产生具有低纤维素分解活性的细胞外木聚糖酶。确定最佳培养条件以获得具有显着木聚糖分解活性和很少纤维素分解活性的粗酶制剂。在大多数处理中，木聚糖分解活性高于纤维素分解活性。随着木聚糖浓度的增加，观察到对内切木聚糖酶、β-木糖苷酶和内切纤维素酶的产生有负面影响。增加温育时间不利地影响内切纤维素酶和β-木糖苷酶的产生。根据数学模型和实验测试，可以生产具有最小内切纤维素酶活性的内切木聚糖酶，增加孵育时间和硫酸铵浓度。F. solani分别为：2.5% (w/v) 木聚糖、5.0、2.0 和 0.4 g/l 酵母提取物、硫酸铵和尿素，孵育 120 小时。