Water stress is the most important adverse factor limiting rice production. Too much water leads to flood and too little leads to drought. Floods and droughts can severely damage crop at different times of the rice life cycle. So the research on submergence tolerance and drought resistance of rice is particularly urgent. In this study, we reported that OsEBP89 (Oryza sativa Ethylene-responsive element binding protein, clone 89), a member of the AP2/ERF subfamily, is involved in a novel signal transduction associated with the tolerance to drought and submergence stress. OsEBP89 was found to be strongly inhibited by drought stress and promoted by submergence. The OsEBP89 protein was located at the nucleus in the rice protoplast. Loss of OsEBP89 was found to improve the seed germination under submerged conditions and also enhanced the tolerance to drought stress throughout growth stage. Additionally, OsEBP89 knockout rice plants increased the accumulation of proline, improved the ability to scavenge ROS compared to overexpression lines and wild type after PEG treatment. Transcriptome data indicates that knockout of OsEBP89 improved the expression of specific genes in response to adverse factors, such as OsAPX1, OsHsfA3, and OsP5CS. Further results indicate that OsEBP89 can interact with and be phosphorylated by SnRK1α (sucrose non-fermenting-1-related protein kinase-1 gene). These findings provide insight into the mechanism of abiotic stress tolerance, and suggest OsEBP89 as a new genetic engineering resource to improve abiotic stress tolerance in rice.

中文翻译：

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APETALA2 /乙烯响应因子OsEBP89敲除可增强对湿地直接播种的适应性，并提高水稻对干旱胁迫的耐受性。

水分胁迫是限制水稻产量的最重要不利因素。太多的水会导致洪水，很少的水会导致干旱。洪水和干旱会在水稻生命周期的不同时间严重损害作物。因此，对水稻的耐淹水性和抗旱性的研究尤为迫切。在这项研究中，我们报道了AP2 / ERF亚家族成员OsEBP89（水稻响应乙烯结合元件，克隆89）与干旱和淹没胁迫的耐受性有关的新型信号转导。发现OsEBP89被干旱胁迫强烈抑制并且被淹没促进。OsEBP89蛋白位于稻原生质体的核中。发现OsEBP89的损失改善了淹没条件下的种子发芽，并且在整个生长期提高了对干旱胁迫的耐受性。此外，与PEG处理后的过表达品系和野生型相比，OsEBP89敲除水稻植株增加了脯氨酸的积累，提高了清除ROS的能力。转录组数据表明，敲除OsEBP89可改善特定基因的表达，以应对不利因素，例如OsAPX1，OsHsfA3和OsP5CS。进一步的结果表明，OsEBP89可与SnRK1α（蔗糖非发酵-1相关蛋白激酶-1基因）相互作用并被其磷酸化。这些发现提供了对非生物胁迫耐受性机制的见解，并建议OsEBP89作为提高水稻非生物胁迫耐受性的新基因工程资源。