Abstract

The potent, selective, and consistent electrochemically polymerized helianthin dye modified carbon paste electrode (PHLDMCPE) was generated for the assessment of the significant antioxidant riboflavin (RL) with ascorbic acid (AA) and tyrosine (TR) using cyclic voltammetric (CV) and differential pulse voltammetric (DPV) techniques. The PHLDMCPE explicates a superior electrocatalytic resolution for the redox activity of 1 × 10⁻⁴ M RL in 0.1 M phosphate buffer solution (PBS) of pH 7.0 than the bare carbon paste electrode (BCPE). The oxidation peak current of RL and concentration of RL were attained linearly in the range of 60 µM to 150 µM with the lower limit of detection (LOD) of 4.02 × 10⁻⁸ M and the limit of quantification (LOQ) of 13.42 × 10⁻⁸ M, respectively. The heterogeneous rate constant for the equipped sensor was detected for RL at variable scan rates. This work conveys a modest and advisory methodology for the concurrent investigation of RL with AA and TR. The studied CV technique and developed sensor were adopted for the assessment of RL in pharmacological medication as a real sample.

Graphic abstract

Electrochemically polymerized HLD modified carbon paste electrode was exposed for the evaluation of antioxidant riboflavin in both real and commercial samples in optimum circumstances using CV and DPV techniques. The selective resolution and interference study of riboflavin were done with ascorbic acid and tyrosine. The surface morphology of modified and unmodified carbon sensors was studied using FE-SEM and CV methods. The modified sensor exposes refined stability, reproducibility, and repeatability with a low LOD (4.02 × 10⁻⁸ M).

中文翻译：

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异质速率常数分析法在聚（堇青素染料）混合碳糊电极上对核黄素的升高和快速伏安感测

摘要

使用循环伏安法（CV）和差示法，生成了有效，选择性和一致的电化学聚合向日葵染料染料修饰的碳糊电极（PHLDMCPE），用于评估抗坏血酸（AA）和酪氨酸（TR）所含的重要抗氧化剂核黄素（RL）脉冲伏安（DPV）技术。PHLDMCPE 对pH 7.0的0.1 M磷酸盐缓冲溶液（PBS）中的1×10 ^-4 M RL的氧化还原活性具有比裸碳糊电极（BCPE）更高的电催化分辨率。RL的氧化峰值电流和RL的浓度在60 µM至150 µM范围内线性获得，检测下限（LOD）为4.02×10 ^-8  M，定量下限（LOQ）为13.42×10 ^-8 M分别。在可变的扫描速率下，检测到配备的传感器的RL的异质速率常数。这项工作为同时研究RL和AA和TR提供了一种适度的咨询方法。所研究的CV技术和开发的传感器被用作药理药物中RL的真实样品评估。

图形摘要

暴露出电化学聚合的HLD修饰的碳糊电极，以在最佳情况下使用CV和DPV技术评估真实和商业样品中的抗氧核黄素。用抗坏血酸和酪氨酸进行了核黄素的选择性拆分和干扰研究。使用FE-SEM和CV方法研究了改性和未改性碳传感器的表面形态。改进后的传感器以低LOD（4.02×10 ^-8  M）展现出精巧的稳定性，可重复性和可重复性。