Odd-carbon fatty acids have been utilized for anaplerotic therapy for lifestyle-related diseases such as Alzheimer’s disease and diabetes. We examined the culture condition of Aurantiochytrium for the production of pentadecanoic acid (C15) as an odd-carbon fatty acid, and demonstrated a produce method for odd-carbon fatty acid–enriched triglyceride. To determine the optimum conditions for C15 production, selection of effective components and their respective concentrations in the culture medium, choice of the suitable Aurantiochytrium strain, and determination of the optimum culture pH were performed.

The optimum conditions for the production of C15 were found to be as follows:

Strain: Aurantiochytrium sp. strain SA-96

Medium composition: 0.2% yeast extract 0.5% monosodium glutamate, 1.0% sea salt, 50 mM L-Val, 25 mM sodium propionate, 3.6% glucose, and 10% soy milk whey.

Culture system: Airlift culture vessels (5.0 L) and pH controller were used. Sodium hydroxide (1.0 M) solution was used for pH adjustment. Other parameters were as follows: medium volume, 3.0 L; air supply, 1.2 vvm; temperature, 23.5–26.6 °C; pH, 7.40–7.74; culture period, 72 h.

The yield of odd-carbon fatty acids (C15, 85% of odd FA) was 1.04 g L⁻¹.

Preparation of odd-carbon fatty acid–enriched triglyceride: Triglyceride was isolated from the extracted lipids from cultured cells and treated with ozone/hydrogen peroxide. By removing of carboxylic acid containing triglyceride, the odd-carbon fatty acid and C15 contents of the resultant triglyceride reached to 72.3 and 60.7%, respectively.

奇碳脂肪酸已被用于与生活方式有关的疾病（例如阿尔茨海默氏病和糖尿病）的过补疗法。我们检查了金葡菌的培养条件，以产生作为奇碳脂肪酸的十五烷酸（C15），并证明了富含奇碳脂肪酸的甘油三酸酯的生产方法。为了确定生产C15的最佳条件，进行了有效成分及其在培养基中的浓度的选择，合适的金眼鱼菌株的选择以及最佳培养pH的确定。

发现生产C15的最佳条件如下：

菌株：Aurantiochytrium sp。SA-96株

中等成分：0.2％酵母提取物，0.5％味精，1.0％海盐，50 mM L-Val，25 mM丙酸钠，3.6％葡萄糖和10％豆乳清。

培养系统：使用Airlift培养皿（5.0 L）和pH控制器。使用氢氧化钠（1.0 M）溶液调节pH。其他参数如下：中等体积，3.0升；气源，1.2 vvm; 温度23.5–26.6°C；pH，7.40–7.74；培养期72小时。

奇碳脂肪酸（C15，85％的奇数FA）的产量为1.04g L ^-1。

富含奇碳脂肪酸的甘油三酸酯的制备：从培养细胞提取的脂质中分离甘油三酸酯，并用臭氧/过氧化氢处理。通过除去包含甘油三酸酯的羧酸，所得甘油三酸酯的奇碳脂肪酸和C15含量分别达到72.3和60.7％。