Lysozyme, a type of natural enzyme, has been widely used for bacteriostatic functionalization of various materials due to its efficient and selective antibacterial properties. Herein, we report the preparation and characterization of an eco-friendly antibacterial textile based on the immobilization of lysozyme from chicken egg white onto wool fibers. Tris(hydroxymethyl)phosphine (THP) was employed as the cross-linker for the immobilization of lysozyme on the surface of wool fiber. The mechanism of THP cross-linking was investigated via phosphorus test, energy-dispersive spectroscopy (EDX) and Fourier transform infrared spectroscopy (FT-IR). The surface staining, optimization of immobilization parameters, morphology, antibacterial properties, and durability of wool fibers with immobilized lysozyme were also assessed. The results show that hydroxymethyl groups of THP reacted with amino groups of wool fiber and lysozyme through Mannich reaction, which successfully immobilized lysozyme on the wool fiber. The wool fibers incorporated with lysozyme had better antibacterial properties and durability compared with the untreated wool fabric. This facile immobilization approach of lysozyme provides an effective strategy for environmentally benign modification and functionalization of keratin and keratin-containing materials.

中文翻译：

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开发一种环保型抗菌纺织品：将溶菌酶固定在羊毛织物上。

溶菌酶是一种天然酶，由于其高效和选择性的抗菌特性，已被广泛用于各种材料的抑菌功能化。在这里，我们报告了基于从鸡蛋白到羊毛纤维的溶菌酶固定化的环保抗菌纺织品的制备和表征。使用三（羟甲基）膦（THP）作为交联剂，将溶菌酶固定在羊毛纤维的表面。通过磷测试，能量色散光谱（EDX）和傅里叶变换红外光谱（FT-IR）研究了THP交联的机理。还评估了固定有溶菌酶的羊毛纤维的表面染色，固定参数的优化，形态，抗菌性能和耐久性。结果表明，THP的羟甲基通过曼尼希反应与羊毛纤维的氨基和溶菌酶反应，成功地将溶菌酶固定在羊毛纤维上。与未处理的羊毛织物相比，掺有溶菌酶的羊毛纤维具有更好的抗菌性能和耐用性。这种简便的溶菌酶固定方法为角蛋白和含角蛋白的材料的环境良性修饰和功能化提供了有效的策略。