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Identification of candidate proteomic markers in the serum of medication overuse headache patients: An exploratory study.
Cephalalgia ( IF 4.9 ) Pub Date : 2020-04-29 , DOI: 10.1177/0333102420921847
Lanfranco Pellesi 1 , Simona Guerzoni 1 , Carlo Baraldi 1 , Maria Michela Cainazzo 1 , Luigi Alberto Pini 1, 2 , Elisa Bellei 3
Affiliation  

PURPOSE OF THE STUDY The pathophysiological mechanism of medication overuse headache is uncertain; no distinctive markers have been described right now. The aim of this study was to conduct proteomic analyses on serum samples from patients with medication overuse headache and healthy individuals. Specifically, mono- (SDS-PAGE) and two-dimensional gel electrophoresis (2-DE) followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to evaluate changes in serum proteins. MAIN FINDINGS By SDS-PAGE, four over-expressed bands were revealed in patients, compared to controls. 2-DE combined with LC-MS/MS analysis allowed confirmation of some proteins preliminarily detected by SDS-PAGE: Hemopexin, alpha-1-acid glycoprotein 1, apolipoprotein A4 and haptoglobin. Moreover, other differential proteins were isolated, mostly increased in MOH patients: Alpha-1-antitrypsin, immunoglobulin heavy constant alpha 1, retinol binding protein and transthyretin. Only one protein, immunoglobulin kappa constant, was decreased in the patients' group. CONCLUSIONS The investigation of the serum proteome can offer a better understanding about biological mechanisms underlying medication overuse headache. Specifically, medication overuse headache shares some serum biochemical markers with chronic pain conditions. Further studies might uncover the relevance of these proteins in medication overuse headache.

中文翻译:

药物过度使用性头痛患者血清中候选蛋白质组学标志物的鉴定:一项探索性研究。

研究目的 药物过度使用性头痛的病理生理机制尚不确定;目前还没有描述任何独特的标记。本研究的目的是对药物过度使用性头痛患者和健康个体的血清样本进行蛋白质组学分析。具体而言,使用单(SDS-PAGE)和二维凝胶电泳(2-DE)和液相色谱串联质谱(LC-MS/MS)来评估血清蛋白的变化。主要发现 通过 SDS-PAGE,与对照相比,在患者中发现了四个过度表达的条带。2-DE 结合 LC-MS/MS 分析可以确认一些通过 SDS-PAGE 初步检测到的蛋白质:血红素结合蛋白、α-1-酸性糖蛋白 1、载脂蛋白 A4 和触珠蛋白。此外,还分离了其他差异蛋白,主要在 MOH 患者中增加:α-1-抗胰蛋白酶、免疫球蛋白重常数α1、视黄醇结合蛋白和转甲状腺素蛋白。患者组中只有一种蛋白质,即免疫球蛋白 kappa 常数下降。结论 血清蛋白质组的研究可以更好地了解药物过度使用性头痛的生物学机制。具体而言,药物过度使用性头痛与慢性疼痛疾病共享一些血清生化标志物。进一步的研究可能会揭示这些蛋白质与药物过度使用性头痛的相关性。结论 血清蛋白质组的研究可以更好地了解药物过度使用性头痛的生物学机制。具体而言,药物过度使用性头痛与慢性疼痛疾病共享一些血清生化标志物。进一步的研究可能会揭示这些蛋白质与药物过度使用性头痛的相关性。结论 血清蛋白质组的研究可以更好地了解药物过度使用性头痛的生物学机制。具体而言,药物过度使用性头痛与慢性疼痛疾病共享一些血清生化标志物。进一步的研究可能会揭示这些蛋白质与药物过度使用性头痛的相关性。
更新日期:2020-04-29
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