Abstract Flaviviruses encompass not only medically relevant arthropod-borne viruses (arboviruses) but also insect-specific flaviviruses (ISFs) that are presumably maintained primarily through vertical transmission in the insect host. Interestingly, ISFs are commonly found infecting important arbovirus vectors such as the mosquito Aedes aegypti. Cell-fusing agent virus (CFAV) was the first described ISF of mosquitoes more than four decades ago. Despite evidence for widespread CFAV infections in A.aegypti populations and for CFAV potential to interfere with arbovirus transmission, little is known about CFAV evolutionary history. Here, we generated six novel CFAV genome sequences by sequencing three new virus isolates and subjecting three mosquito samples to untargeted viral metagenomics. We used these new genome sequences together with published ones to perform a global phylogenetic analysis of CFAV genetic diversity. Although there was some degree of geographical clustering among CFAV sequences, there were also notable discrepancies between geography and phylogeny. In particular, CFAV sequences from Cambodia and Thailand diverged significantly, despite confirmation that A.aegypti populations from both locations are genetically close. The apparent phylogenetic discrepancy between CFAV and its A.aegypti host in Southeast Asia indicates that other factors than host population structure shape CFAV genetic diversity.

中文翻译：

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细胞融合剂病毒的新基因组序列允许将病毒系统发育与埃及伊蚊种群的遗传结构进行比较

摘要 黄病毒不仅包括医学上相关的节肢动物传播病毒（虫媒病毒），还包括昆虫特异性黄病毒（ISF），它们可能主要通过昆虫宿主中的垂直传播来维持。有趣的是，ISF 通常会感染重要的虫媒病毒载体，例如埃及伊蚊。细胞融合剂病毒 (CFAV) 是四十多年前首次描述的蚊子 ISF。尽管有证据表明埃及伊蚊种群中广泛存在 CFAV 感染，并且 CFAV 有可能干扰虫媒病毒传播，但对 CFAV 的进化历史知之甚少。在这里，我们通过对三个新病毒分离株进行测序并对三个蚊子样本进行非靶向病毒宏基因组学分析，生成了六个新的 CFAV 基因组序列。我们将这些新的基因组序列与已发表的序列一起使用，对 CFAV 遗传多样性进行了全球系统发育分析。尽管 CFAV 序列之间存在一定程度的地理聚类，但地理和系统发育之间也存在显着差异。特别是，来自柬埔寨和泰国的 CFAV 序列显着不同，尽管确认来自这两个地点的埃及 A.aegypti 种群在遗传上很接近。CFAV 与其在东南亚的 A.aegypti 宿主之间明显的系统发育差异表明，除了宿主种群结构之外，其他因素也影响了 CFAV 的遗传多样性。特别是，来自柬埔寨和泰国的 CFAV 序列显着不同，尽管确认来自这两个地点的埃及 A.aegypti 种群在遗传上很接近。CFAV 与其在东南亚的 A.aegypti 宿主之间明显的系统发育差异表明，除了宿主种群结构之外，其他因素也影响了 CFAV 的遗传多样性。特别是，来自柬埔寨和泰国的 CFAV 序列显着不同，尽管确认来自这两个地点的埃及 A.aegypti 种群在遗传上很接近。CFAV 与其在东南亚的 A.aegypti 宿主之间明显的系统发育差异表明，除了宿主种群结构之外，其他因素也影响了 CFAV 的遗传多样性。