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Spatial frequency modulated imaging in coherent anti-Stokes Raman microscopy
Optica ( IF 10.4 ) Pub Date : 2020-05-01 , DOI: 10.1364/optica.386526
Sandro Heuke 1 , Siddharth Sivankutty 1 , Camille Scotte 1 , Patrick Stockton 2 , Randy A Bartels 2 , Anne Sentenac 1 , Hervé Rigneault 1
Affiliation  

For sparse samples or in the presence of ambient light, the signal-to-noise ratio (SNR) performance of single-point-scanning coherent anti-Stokes Raman scattering (CARS) images is not optimized. As an improvement, we propose replacing the conventional CARS focus-point illumination with a periodically structured focus line while continuing to collect the transmitted CARS intensity on a single detector. The object information along the illuminated line is obtained by numerically processing the CARS signal recorded for various periods of the structured focus line. We demonstrate experimentally the feasibility of this spatial frequency modulated imaging (SPIFI) in CARS (SPIFI-CARS) and SHG (SPIFI-SHG) and identify situations where its SNR is better than that of the single-point-scanning approach.

中文翻译:

相干反斯托克斯拉曼显微镜中的空间频率调制成像

对于稀疏样本或存在环境光的情况,单点扫描相干反斯托克斯拉曼散射 (CARS) 图像的信噪比 (SNR) 性能未优化。作为一项改进,我们建议用周期性结构的聚焦线代替传统的 CARS 焦点照明,同时继续在单个检测器上收集透射的 CARS 强度。沿着照明线的物体信息是通过对结构化聚焦线的各个周期记录的 CARS 信号进行数值处理而获得的。我们通过实验证明了这种空间频率调制成像 (SPIFI) 在 CARS (SPIFI-CARS) 和 SHG (SPIFI-SHG) 中的可行性,并确定了其 SNR 优于单点扫描方法的情况。
更新日期:2020-05-01
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