Melanoma is a skin cancer with high invasive potential and high lethality. Considering that quinonemethide triterpenes are described as promising anticancer agents, the aim of this study was to evaluate the effect of 22β-hydroxytingenone (22-HTG) against human melanoma cells. Alamar blue assay was performed in order to evaluate its cytotoxic effect. Thus, subtoxic concentrations (1.0, 2.0, and 2.5 μM) were used to evaluate the effect of this compound on proliferation, migration, metabolism, and invasion. IC50 value against SK-MEL-28 cell line was 4.35, 3.72, and 3.29 μM after 24, 48, and 72 h of incubation, respectively. 22-HTG reduced proliferation, migration and invasion by melanoma cells, with decreased activity of metalloproteinases (MMP-2 and MMP-9). Futhermore, 22-HTG decreased expression of lactate dehydrogenase (LDHA), an enzyme associated with cell metabolism. Howerver, the small reduction in LDHA enzyme activity must have occurred by the cytotoxic effect of 22-HTG. According to the results, 22-HTG interferes with important characteristics of cancer, with anti-proliferative, and anti-invasive effect against melanoma cells. The data suggest that 22-HTG is an effective substance against melanoma cells and it should be considered as a potential anticancer agent.

中文翻译：

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22β-羟基丁烯酮减少人黑素瘤细胞的增殖和侵袭。

黑色素瘤是一种具有高浸润性和高致死性的皮肤癌。考虑到醌甲基三萜烯被描述为有前途的抗癌药，本研究的目的是评估22β-羟基丁烯酮（22-HTG）对人黑素瘤细胞的作用。为了评估其细胞毒性作用，进行了Alamar蓝分析。因此，亚毒性浓度（1.0、2.0和2.5μM）用于评估该化合物对增殖，迁移，代谢和侵袭的影响。孵育24、48和72小时后，对SK-MEL-28细胞系的IC50值分别为4.35、3.72和3.29μM。22-HTG减少了黑色素瘤细胞的增殖，迁移和侵袭，同时降低了金属蛋白酶（MMP-2和MMP-9）的活性。此外，22-HTG降低了乳酸脱氢酶（LDHA）的表达，与细胞代谢有关的酶。但是，由于22-HTG的细胞毒性作用，LDHA酶的活性必须有所降低。根据结果​​，22-HTG干扰了癌症的重要特征，具有针对黑素瘤细胞的抗增殖和抗侵袭作用。数据表明22-HTG是对抗黑色素瘤细胞的有效物质，应将其视为潜在的抗癌剂。