A new trisnorlanostene-type triterpenoid 25,26,27-trisnor-3α-hydroxy-lanost-7(8)-en-24-oic acid and a new isoflavone, formononetin-7-O-[6″-O-((S)-3-hydroxy-3-methylglutaryl)]-β-d-glucopyranoside, in addition to twelve known compounds, were isolated from the ethyl acetate and n-butanolic extracts of Ononis mitissima L. Their structures were elucidated by spectroscopic analysis, including 1D and 2D NMR, mass spectrometry and comparison with literature data. The total phenolic and flavonoid contents were estimated. Furthermore, the antihemolytic and anti-inflammatory activities of the crude extracts (petroleum ether, ethyl acetate and n-butanol) were evaluated, as well as their antioxidant activity. The tested extracts displayed antioxidant activity on different methods including DPPH free radical scavenging activity, ferric reducing antioxidant power, ferric thiocyanate assay, hydrogen peroxide scavenging capacity and total antioxidant activity by phosphomolybdate assay. All extracts showed a moderate anti-inflammatory activity at the concentration of 500 μg/mL. The strongest antihemolytic activity was observed with the EtOAc extract.

一个新的tris或lanostene型三萜25,26,27-tris或-3α-hydroxy-lanost-7（8）-en-24-oic acid和一个新的异黄酮，formononetin-7- O- [6″ -O除了十二种已知化合物以外，还从Ononis mitissima的乙酸乙酯和正丁醇提取物中分离了-（（（S）-3-羟基-3-甲基戊二芳基）]-β- d-吡喃葡萄糖苷L.通过光谱分析（包括1D和2D NMR，质谱法以及与文献数据的比较）阐明了它们的结构。估计总酚和类黄酮含量。此外，粗提物的抗溶血和抗炎活性（石油醚，乙酸乙酯和Ñ-丁醇）及其抗氧化活性进行了评估。所测试的提取物在不同的方法上表现出抗氧化活性，包括DPPH自由基清除活性，三氧化二铁还原能力，硫氰酸铁测定，过氧化氢清除能力和磷钼酸盐测定的总抗氧化活性。所有提取物在500μg/ mL的浓度下均显示出中等的抗炎活性。用EtOAc提取物观察到最强的抗溶血活性。