Background

Atopic dermatitis (AD) and food allergy (FA) are associated with skin barrier dysfunction.

Objective

Skin biomarkers are needed for skin barrier interventions studies.

Methods

In this study, skin tape strip (STS) samples were collected from nonlesional skin of 62 children in AD FA+, AD FA−, and nonatopic groups for mass spectrometry proteomic analysis. transepidermal water loss and allergic sensitization were assessed. STS proteomic analysis results were validated in an independent cohort of 41 adults with AD with and without FA versus nonatopic controls.

Results

A group of 45 proteins was identified as a principal component 1 (PC1) with the highest expression in AD FA+ STSs. This novel set of STS proteins was highly correlative to skin transepidermal water loss and allergic sensitization. PC1 proteins included keratin intermediate filaments; proteins associated with inflammatory responses (S100 proteins, alarmins, protease inhibitors); and glycolysis and antioxidant defense enzymes. Analysis of PC1 proteins expression in an independent adult AD cohort validated differential expression of STS PC1 proteins in the skin of adult patients with AD with the history of clinical reactions to peanut.

Conclusions

STS analysis of nonlesional skin of AD children identified a cluster of proteins with the highest expression in AD FA+ children. The differential expression of STS PC1 proteins was confirmed in a replicate cohort of adult AD patients with FA to peanut, suggesting a unique STS proteomic endotype for AD FA+ that persists into adulthood. Collectively, PC1 proteins are associated with abnormalities in skin barrier integrity and may increase the risk of epicutaneous sensitization to food allergens.

中文翻译：

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皮肤胶带蛋白质组学确定了与特应性皮炎中经表皮水分流失和过敏原多敏化相关的途径。

背景

特应性皮炎 (AD) 和食物过敏 (FA) 与皮肤屏障功能障碍有关。

客观的

皮肤屏障干预研究需要皮肤生物标志物。

方法

在这项研究中，从 AD FA +、 AD FA -和非特应性组的 62 名儿童的非损伤皮肤中收集皮肤胶带 (STS) 样本，用于质谱蛋白质组学分析。评估了经皮水分流失和过敏性致敏性。STS 蛋白质组学分析结果在一个独立队列中得到验证，该队列由 41 名伴有和不伴有 FA 的 AD 成人与非特应性对照者组成。

结果

一组 45 种蛋白质被鉴定为在 AD FA + STS中表达最高的主要成分 1 (PC1) 。这组新的 STS 蛋白与皮肤经表皮水分流失和过敏致敏高度相关。PC1 蛋白包括角蛋白中间丝；与炎症反应相关的蛋白质（S100 蛋白、警报素、蛋白酶抑制剂）；和糖酵解和抗氧化防御酶。对独立成人 AD 队列中 PC1 蛋白表达的分析验证了 STS PC1 蛋白在具有花生临床反应史的成人 AD 患者皮肤中的差异表达。

结论

对 AD 儿童非病变皮肤的 STS 分析确定了一组在 AD FA +儿童中表达最高的蛋白质。STS PC1 蛋白的差异表达在 FA 到花生的成年 AD 患者的重复队列中得到证实，表明 AD FA+ 的独特 STS 蛋白质组内型持续到成年期。总的来说，PC1 蛋白与皮肤屏障完整性异常有关，并可能增加对食物过敏原的表皮致敏风险。