African swine fever virus (ASFV) is a complex double stranded DNA virus, responsible for a highly infectious and fatal disease in pigs and boars and for important deterioration of animal welfare. Over the last decade, the disease spread to several European and Asian countries causing unprecedented dramatic economic losses in pig industry. In the absence of a vaccine, affected countries rely on trustful diagnostic tests and adapted testing policies to set up control programs to fight against the disease. In this study, we evaluated the sensitivity and specificity of seven commercially available ASFV real-time PCR detection kits and three Taq polymerases on 300 well-characterized wild boar samples collected in Belgium during the 2018-2019 outbreak. This study confirms that all commercial kits and two Taq polymerases are suitable for ASFV detection in diagnostic laboratories. Furthermore, the use of endogenous controls is emphasized when testing field samples harvested on carcasses in an advanced stage of decomposition, in order to avoid false negative results.

中文翻译：

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在300个特征明确的野外样品上评估了七个商业化的非洲猪瘟病毒检测试剂盒和三个Taq聚合酶。

非洲猪瘟病毒（ASFV）是一种复杂的双链DNA病毒，在猪和公猪中引起高度传染性和致命性疾病，并严重破坏了动物的福利。在过去的十年中，该病传播到几个欧洲和亚洲国家，给养猪业造成了空前的巨大经济损失。在没有疫苗的情况下，受影响的国家依靠可靠的诊断测试和适应的测试政策来建立控制方案来对抗这种疾病。在这项研究中，我们评估了7种市售ASFV实时PCR检测试剂盒和3种Taq聚合酶对2018-2019年比利时暴发期间在比利时收集的300个特征充分的野猪样品的敏感性和特异性。这项研究证实，所有商业试剂盒和两种Taq聚合酶均适用于诊断实验室中的ASFV检测。此外，当测试在分解后期进入屠体的田间样品时，应强调使用内源性对照，以避免假阴性结果。